GSK3 beta (Phospho) [pS9] Multispecies ELISA Kit

Catalog number: EMS2GSK3P

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

Assay phosphorylated GSK-3β ([pSer9] glycogen synthase kinase 3 beta, pGSK-3β) levels in cell lysates of human, mouse, and rat origin with our Thermo Scientific Phospho GSK-3 beta ELISA Kit.

Features of the Phospho GSK-3 beta ELISA Kit:

Target: phosphorylated GSK-3β ([pSer9] glycogen synthase kinase 3 beta, pGSK-3β)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 62.5 to 2000 pg/mL
Sensitivity: < 9 pg/mL
Precision: dependent upon sample type and level (see instructions)
Sample Types: cell lysates of human, mouse, and rat origin
Total Assay Time: 3 hours
Sample Size: 100 µL
Specificity: no significant cross-reaction with GSK-3α, ATP Citrate Lyase, MEK, AKT, phospho-JNK, ERK and phospho-ERK.

This phospho-GSK-3 beta ELISA kit is an immunoassay for the quantitative determination of phosphorylated glycogen synthase kinase 3 beta (pGSK-3β) in cell lysates. A recombinant GSK-3β standard is provided with the kit. The assay uses a monoclonal antibody to GSK-3β precoated in a microplate to bind the GSK-3β in the standards or sample. After a short incubation, the excess sample or standard is washed out of the plate and a phospho-GSK-3b detection antibody is added. This antibody binds to the pGSK-3β captured on the plate. After a short incubation, the excess antibody is washed out and secondary conjugate is added, which binds to the phospho-GSK-3β detection antibody. Excess conjugate is washed out and TMB substrate is added. After a short incubation, the enzyme reaction is stopped and the absorbance is read at 450nm. The measured optical density is directly proportional to the concentration of pGSK-3β in either standards or samples.

Includes:
Complete kit contains pre-coated antibody-plate, detection antibodies, buffers, standard, substrate, and stop solution.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene ID: 2932
Gene Name: glycogen synthase kinase 3 beta
Gene Symbol: GSK3B
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Aliases: GSK-3 beta, Serine/threonine-protein kinase GSK3B
Protein Name: Glycogen synthase kinase-3 beta
Target (Kit Named): GSK3 beta
Target Site-Specificity: [pS9]
Target Species (Named): Multispecies
Target Species (Validated): Human, Mouse, Rat
Target Sub-Specificity: Phospho
UniProt ID: P49841

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols