MEK 1 (total) ELISA Kit, Multispecies

Catalog number: EMS2MEKT

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Assay total MEK-1 (pan MAPK-ERK kinase 1, MEK1 + phosho-MEK1) levels in cell lysates of human, mouse, and rat origin with our Thermo Scientific Total MEK-1 ELISA Kit.

Features of the Total MEK-1 ELISA Kit:

Target: total MEK-1 (pan MAPK-ERK kinase 1, MEK1 + pMEK1)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 312.5 to 10,000 pg/mL
Sensitivity: < 139 pg/mL
Precision: dependent upon sample type and level (see instructions)
Sample Types: cell lysates of human, mouse, and rat origin
Total Assay Time: 3 hours
Sample Size: 100 µL
Specificity: no significant cross-reaction with phospho-JNK, ERK2, GSK-3b and p38; approx. 17% cross-reaction with phospho-MEK2.

This MEK1 ELISA kit is an immunoassay for the quantitative determination of pan or total MEK1 in cell lysates. A recombinant phosphorylated MEK1 standard is provided in the kit. The kit uses a monoclonal antibody to MEK1 precoated in a microplate to bind the MEK1 in the standards or samples. After a short incubation the excess sample or standard is washed out and a MEK1/2 detection antibody is added. This antibody binds to the MEK1 captured on the plate. After a short incubation the excess antibody is washed out and secondary conjugate is added, which binds to the MEK1/2 detection antibody. Excess conjugate is washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450nm. The measured optical density is directly proportional to the concentration of MEK1 in either standards or samples.

Includes:
Complete kit contains pre-coated antibody-plate, detection antibodies, buffers, standard, substrate, and stop solution.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Chemically Competent
Species: E. coli
Format: Tube(s)
Transformation Efficiency: > 1x10^9
Bacterial or Yeast Strain: DH5α™
Product Size: 10 reactions
Blue-White Screening: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Reduces Recombination: Yes
Cloning Methylated DNA: No
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Lacks F' Episome
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): No
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Shipping Condition: Dry Ice

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols