Cyclic GMP Competitive ELISA Kit, Multispecies

Catalog number: EMSCGMPL

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Assay cyclic GMP (cGMP) levels in culture supernatant, cell lysate and tissue homogenate samples (treated with 0.1M HCl and acetylated or left non-acetylated) using our Thermo Scientific Cyclic GMP Competitive ELISA Kit.

Features of the Cyclic GMP Competitive ELISA:

Target: cyclic guanosine monophosphate (cyclic GMP, cGMP)
Format: colorimetric ELISA (p-NPP substrate) in clear 96-well plate
Measurement: absorbance at 405 nm minus absorbance at 570 nm
Assay Range: 0.8 to 500 pmol/mL (non-acetylated); 0.08 to 50 pmol/mL (acetylated)
Sensitivity: 0.6 pmol/mL (non-acetylated); 0.06 pmol/mL (acetylated)
Precision: dependent upon sample type, level and assay (see instructions)
Sample Types: cell culture supernatant, cell lysate, tissue homogenate
Total Assay Time: 3 hours (non-acetylated); 2 hours (acetylated)
Sample Size: 100 µL
Specificity: no significant cross-reaction with GMP, GTP, cAMP, AMP, ATP, cUMP, or CTP

This cyclic GMP competitive ELISA kit is an immunoassay for the quantitative determination of cyclic guanosine monophosphate (cGMP) in samples treated with 0.1M HCl. The assay is based on the competition between cGMP in the standard or sample and alkaline phosphatase conjugated cGMP (cGMP-AP) for a limited amount of cGMP monoclonal antibody bound to an anti-rabbit IgG precoated 96-well plate. As the concentration of cGMP in the sample increases, the amount of cGMP-AP captured by the coating antibody decreases. Thus, there is an inverse relationship between optical density (OD) and the amount of analyte in the sample.
For Research Use Only. Not for use in diagnostic procedures.


Species: Multiple
Product Size: 96 assays
Protein Family: Other Proteins
Detection Method: Colorimetric
Research Category: Signal Transduction

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols