Cyclic GMP Multispecies Competitive ELISA Kit

      Cyclic GMP Multispecies Competitive ELISA Kit

      Catalog number:  EMSCGMPL

      Invitrogen™  Related applications: Protein Assays and Analysis

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      Description

      Assay cyclic GMP (cGMP) levels in culture supernatant, cell lysate and tissue homogenate samples (treated with 0.1M HCl and acetylated or left non-acetylated) using our Thermo Scientific Cyclic GMP Competitive ELISA Kit.

      Features of the Cyclic GMP Competitive ELISA:

      • Target: cyclic guanosine monophosphate (cyclic GMP, cGMP)
      • Format: colorimetric ELISA (p-NPP substrate) in clear 96-well plate
      • Measurement: absorbance at 405 nm minus absorbance at 570 nm
      • Assay Range: 0.8 to 500 pmol/mL (non-acetylated); 0.08 to 50 pmol/mL (acetylated)
      • Sensitivity: 0.6 pmol/mL (non-acetylated); 0.06 pmol/mL (acetylated)
      • Precision: dependent upon sample type, level and assay (see instructions)
      • Sample Types: cell culture supernatant, cell lysate, tissue homogenate
      • Total Assay Time: 3 hours (non-acetylated); 2 hours (acetylated)
      • Sample Size: 100 µL
      • Specificity: no significant cross-reaction with GMP, GTP, cAMP, AMP, ATP, cUMP, or CTP

      This cyclic GMP competitive ELISA kit is an immunoassay for the quantitative determination of cyclic guanosine monophosphate (cGMP) in samples treated with 0.1M HCl. The assay is based on the competition between cGMP in the standard or sample and alkaline phosphatase conjugated cGMP (cGMP-AP) for a limited amount of cGMP monoclonal antibody bound to an anti-rabbit IgG precoated 96-well plate. As the concentration of cGMP in the sample increases, the amount of cGMP-AP captured by the coating antibody decreases. Thus, there is an inverse relationship between optical density (OD) and the amount of analyte in the sample.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Protein Family: Other Proteins
      Assay Range: See product documentation
      Assay Sensitivity: See product documentation
      Detection Method: Colorimetric
      For Use With (Equipment): Microplate Reader
      Former Identifiers: Pierce
      Gene ID: Non-Gene
      Gene Symbol: Non-Gene
      Immunoassay Kit Format: Competitive ELISA Kit
      Label or Dye: HRP (Horseradish Peroxidase)
      Product Size: 96 tests
      Target (Kit Named): Cyclic GMP
      Target Species (Named): Multispecies
      Target Species (Validated): Human

      Contents & storage

      Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

      Documents

      Manuals & protocols