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Cyclic GMP Multispecies Competitive ELISA Kit

Catalog number: EMSCGMPL

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

Assay cyclic GMP (cGMP) levels in culture supernatant, cell lysate and tissue homogenate samples (treated with 0.1M HCl and acetylated or left non-acetylated) using our Thermo Scientific Cyclic GMP Competitive ELISA Kit.

Features of the Cyclic GMP Competitive ELISA:

Target: cyclic guanosine monophosphate (cyclic GMP, cGMP)
Format: colorimetric ELISA (p-NPP substrate) in clear 96-well plate
Measurement: absorbance at 405 nm minus absorbance at 570 nm
Assay Range: 0.8 to 500 pmol/mL (non-acetylated); 0.08 to 50 pmol/mL (acetylated)
Sensitivity: 0.6 pmol/mL (non-acetylated); 0.06 pmol/mL (acetylated)
Precision: dependent upon sample type, level and assay (see instructions)
Sample Types: cell culture supernatant, cell lysate, tissue homogenate
Total Assay Time: 3 hours (non-acetylated); 2 hours (acetylated)
Sample Size: 100 µL
Specificity: no significant cross-reaction with GMP, GTP, cAMP, AMP, ATP, cUMP, or CTP

This cyclic GMP competitive ELISA kit is an immunoassay for the quantitative determination of cyclic guanosine monophosphate (cGMP) in samples treated with 0.1M HCl. The assay is based on the competition between cGMP in the standard or sample and alkaline phosphatase conjugated cGMP (cGMP-AP) for a limited amount of cGMP monoclonal antibody bound to an anti-rabbit IgG precoated 96-well plate. As the concentration of cGMP in the sample increases, the amount of cGMP-AP captured by the coating antibody decreases. Thus, there is an inverse relationship between optical density (OD) and the amount of analyte in the sample.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Other Proteins
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene ID: Non-Gene
Gene Symbol: Non-Gene
Immunoassay Kit Format: Competitive ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Target (Kit Named): Cyclic GMP
Target Species (Named): Multispecies
Target Species (Validated): Human

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols