JNK1/JNK2 (Phospho) [pT183/pY185] Multispecies ELISA Kit

Catalog number: EMSJNKP

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

Assay phosphorylated JNK-1 and JNK-2 ([pThr183/Tyr185] c-Jun N-terminal protein kinase, pJNK) levels in cell lysates of human, mouse, and rat origin with our Thermo Scientific Phospho JNK 1/2 ELISA Kit.

Features of the Phospho JNK 1/2 ELISA Kit:

Target: phosphorylated JNK-1 and JNK-2 ([pThr183/Tyr185] c-Jun N-terminal protein kinase, pJNK)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 125 to 4000 pg/mL
Sensitivity: < 75.8 pg/mL
Precision: dependent upon sample type and level (see instructions)
Sample Types: cell lysates of human, mouse, and rat origin
Total Assay Time: 3 hours
Sample Size: 100 µL
Specificity: no significant cross-reaction with non-phosphorylated ERK2, phospho-ERK2, phospho-p38, non-phosphorylated JNK1.

This phospho-JNK ELISA kit is an immunoassay for the quantitative determination of phosphorylated c-Jun N-terminal protein kinase (pJNK, pJNK 1/2) in cell lysates. A recombinant pJNK1 Standard is provided with the kit. The assay uses a monoclonal antibody to JNK precoated in a microplate to bind the JNK in the standards or sample. After a short incubation, the excess sample or standard is washed out of the plate and a biotinylated phospho-JNK detection antibody is added. This antibody binds to the pJNK captured on the plate. After a short incubation, the excess antibody is washed out and secondary conjugate is added, which binds to the biotinylated phospho JNK detection antibody. Excess conjugate is washed out and TMB substrate is added. After a short incubation, the enzyme reaction is stopped and the absorbance is read at 450nm. The measured optical density is directly proportional to the concentration of pJNK in either standards or samples.

Includes:
Complete kit contains pre-coated antibody-plate, detection antibodies, buffers, standard, substrate, and stop solution.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c, JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, SAPK1a, p54a, p54aSAPK
Gene ID: 5599, 5601
Gene Name: mitogen-activated protein kinase 8, mitogen-activated protein kinase 9
Gene Symbol: MAPK8, MAPK9
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Name: Mitogen-activated protein kinase 8, Mitogen-activated protein kinase 9
Target (Kit Named): JNK1/JNK2
Target Site-Specificity: [pT183/pY185]
Target Species (Named): Multispecies
Target Species (Validated): Human, Mouse, Rat
Target Sub-Specificity: Phospho
UniProt ID: P45983, P45984

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols