JNK 1/2 [pT183/pY185] Phospho-ELISA Kit, Multispecies

Catalog number: EMSJNKP

Thermo Scientific™  Related applications: Protein Assays and Analysis

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Assay phosphorylated JNK-1 and JNK-2 ([pThr183/Tyr185] c-Jun N-terminal protein kinase, pJNK) levels in cell lysates of human, mouse, and rat origin with our Thermo Scientific Phospho JNK 1/2 ELISA Kit.

Features of the Phospho JNK 1/2 ELISA Kit:

Target: phosphorylated JNK-1 and JNK-2 ([pThr183/Tyr185] c-Jun N-terminal protein kinase, pJNK)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 125 to 4000 pg/mL
Sensitivity: < 75.8 pg/mL
Precision: dependent upon sample type and level (see instructions)
Sample Types: cell lysates of human, mouse, and rat origin
Total Assay Time: 3 hours
Sample Size: 100 µL
Specificity: no significant cross-reaction with non-phosphorylated ERK2, phospho-ERK2, phospho-p38, non-phosphorylated JNK1.

This phospho-JNK ELISA kit is an immunoassay for the quantitative determination of phosphorylated c-Jun N-terminal protein kinase (pJNK, pJNK 1/2) in cell lysates. A recombinant pJNK1 Standard is provided with the kit. The assay uses a monoclonal antibody to JNK precoated in a microplate to bind the JNK in the standards or sample. After a short incubation, the excess sample or standard is washed out of the plate and a biotinylated phospho-JNK detection antibody is added. This antibody binds to the pJNK captured on the plate. After a short incubation, the excess antibody is washed out and secondary conjugate is added, which binds to the biotinylated phospho JNK detection antibody. Excess conjugate is washed out and TMB substrate is added. After a short incubation, the enzyme reaction is stopped and the absorbance is read at 450nm. The measured optical density is directly proportional to the concentration of pJNK in either standards or samples.

Includes:
Complete kit contains pre-coated antibody-plate, detection antibodies, buffers, standard, substrate, and stop solution.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Multiple
Product Size: 96 assays
Protein Family: Other Proteins
Detection Method: Colorimetric
Research Category: Signal Transduction

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols