Nitric Oxide (Total) Multispecies ELISA Kit

Catalog number:  EMSNOTOT

Invitrogen™  Related applications: Protein Assays and Analysis

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Assay nitric oxide (NO) composition and total as separate values of nitrate (NO3-) and nitrite (NO2-) levels in urine, saliva, plasma, serum and other biological fluids with our Thermo Scientific Nitric Oxide Assay Kit.

Features of the Nitric Oxide Assay Kit:

Target: total nitric oxide (NO) contributed by the sum of nitrite (NO2-) and nitrate (NO3-)
Format: colorimetric stain (azo dye) for clear 96-well plates
Measurement: absorbance at 540nm (±20nm)
Assay Range: 3.1 to 100μM
Precision: dependent upon several factors (generally, intra- and inter-assay CV ≤ 5%)
Sample Types: urine, saliva, plasma, serum and other biological fluids
Total Assay Time: 45 minutes
Sample Size: 50 µL

This nitric oxide assay kit is for quantitative determination of nitrite (NO2-) and nitrate (NO3-) in biological fluids. The kit uses the enzyme nitrate reductase to convert nitrate to nitrite. Nitrite is then detected as a colored azo dye product of the Griess reaction that absorbs visible light at 540nm. Total nitric oxide (NO) contributed by nitrate and nitrite in a system is measured as nitrite after converting all nitrate to nitrite.

Complete kit contains Griess reagents, nitrite and nitrate standards, nitrate reductase, NADH, and diluents.

In this kit, all of the nitrate in a sample is converted to nitrite using nitrate reductase enzyme and the total amount of nitrite (pre-existing nitrite plus nitrate that was converted) is measured. The kit contains sufficient reagents for 192 determinations of nitrite and is designed for researchers looking for the overall concentration of nitric oxide in each sample.
For Research Use Only. Not for use in diagnostic procedures.


Protein Family: Other Proteins
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene ID: Non-Gene
Gene Symbol: Non-Gene
Immunoassay Kit Format: Detection Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 2 x 96 tests
Target (Kit Named): Nitric Oxide
Target Species (Named): Multispecies
Target Species (Validated): Human
Target Sub-Specificity: Total

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols