Thermo Scientific Micrococcal Nuclease (S7 Nuclease) is an endo-exonuclease that digests single-stranded and double-stranded DNA and RNA. Micrococcal Nuclease (S7 Nuclease) is a relatively nonspecific endo-exonuclease that digests single-stranded and double-stranded nucleic acids, but is more active on single-stranded substrates. Cleavage of DNA or RNA occurs preferentially at AT or AU-rich regions, yielding mononucleotides and oligonucleotides with terminal 3'-phosphates. The enzyme's activity is strictly dependent on Ca2+. Applications
• Hydrolysis of nucleic acids in crude cell-free extracts • Sequencing of RNA (see Reference 2) • Studies of chromatin structure • A model for protein folding and for structure-function studies
For Research Use Only. Not for use in diagnostic procedures.