Thermo Scientific™

Micrococcal Nuclease (300 U/µL)

Catalog number:  EN0181

Related applications:

Nucleic Acid Gel Electrophoresis & Blotting

| Chromatin Biology

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Description

Thermo Scientific Micrococcal Nuclease (S7 Nuclease) is an endo-exonuclease that digests single-stranded and double-stranded DNA and RNA.
Micrococcal Nuclease (S7 Nuclease) is a relatively nonspecific endo-exonuclease that digests single-stranded and double-stranded nucleic acids, but is more active on single-stranded substrates. Cleavage of DNA or RNA occurs preferentially at AT or AU-rich regions, yielding mononucleotides and oligonucleotides with terminal 3'-phosphates. The enzyme's activity is strictly dependent on Ca2+.
Applications

• Hydrolysis of nucleic acids in crude cell-free extracts
• Sequencing of RNA (see​ Reference 2)
• Studies of chromatin structure
• A model for protein folding and for structure-function studies
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type: Micrococcal Nuclease
Concentration: 300 U/µL
Quantity: 8000 unit
Enzyme: Nuclease