Thermo Scientific™

RNase H (5 U/µL)

Catalog number:  EN0201

Related applications:

Real Time PCR (qPCR)

| Reverse Transcription

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Description

Thermo Scientific Ribonuclease H (RNase H) specifically degrades the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.

Applications

• Removal of mRNA prior to synthesis of second strand cDNA
RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis
• Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT)
• Site-specific cleavage of RNA
• Studies of in vitro polyadenylation reaction products
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type: RNase H
Concentration: 5 U/µL
Quantity: 100U
Enzyme: RNase
Compatible Buffer: 10X Reaction Buffer