Thermo Scientific™

Uracil-DNA Glycosylase (1 U/µL)

Catalog number:  EN0361

Related applications:

PCR

| Real Time PCR (qPCR)

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Description

Thermo Scientific Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA (see Figure 1 in Supporting Data). The enzyme shows no activity on RNA.

Highlights

Active in Thermo Scientific buffers for restriction enzymes and thermophilic polymerases

Applications

• Control of carry-over contamination in PCR
• Glycosylase mediated single nucleotide polymorphism detection (GMPD)
• Site-directed mutagenesis
• As a probe for protein-DNA interaction studies
• SNP genotyping
• Cloning of PCR products
• Generation of single strand overhangs of PCR products and cDNA

Note

The abasic sites formed in DNA by Uracil-DNA Glycosylase may be subsequently cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites. UDG (UNG) is active in the presence or absence of divalent cations.

Use of this enzyme in certain applications may be covered by patents and may require a license.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Enzyme: Uracil-DNA Glycosylase
Quantity: 200U

Contents & storage

Uracil-DNA glycolase (1 U/µL) and 10X Reaction Buffer. Store at -20°C.