RNase A, DNase and protease-free (10 mg/mL)
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RNase A, DNase and protease-free (10 mg/mL)
Thermo Scientific™

RNase A, DNase and protease-free (10 mg/mL)

Thermo Scientific RNase A, DNase and protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues.ItRead more
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Catalog number EN0531
Price (USD)/ Each
51.65
Online exclusive
61.75 
Save 10.10 (16%)
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Add to cart
Request bulk or custom format
Price (USD)/ Each
51.65
Online exclusive
61.75 
Save 10.10 (16%)
Add to cart
RNase A, DNase and protease-free (10 mg/mL)
Catalog numberEN0531
Price (USD)/ Each
51.65
Online exclusive
61.75 
Save 10.10 (16%)
-
Add to cart

Thermo Scientific RNase A, DNase and protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues.

It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate.

Highlights

  • RNase A is free of DNase activity. It is not necessary to heat it before use.

Applications

  • Plasmid and genomic DNA preparation
  • Removal of RNA from recombinant protein preparations
  • Ribonuclease protection assays. Used in conjunction with RNase T1
  • Mapping single-base mutations in DNA or RNA

Notes

Recommended concentration of RNase A is 1 to 100 μg/mL depending on the application. The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration10 mg/mL
EnzymeRNase
Quantity10 mg
Product TypeRNase A
Unit SizeEach
Contents & Storage
Store at -20°C.