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Thermo Scientific™

Exonuclease I (20 U/µL)

Catalog number: EN0582
Thermo Scientific™

Exonuclease I (20 U/µL)

Catalog number: EN0582
Catalog Number
EN0582
Unit Size
20,000 units
Price (USD)
Price: 432.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
4,000 units
Price (USD)
Price: 112.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EN058220,000 units
Price: 432.00
Your Price:
-
EN05814,000 units
Price: 112.00
Your Price:
-
Product Overview
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Additional Information
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Thermo Scientific Exonuclease I (ExoI) degrades single-stranded DNA in a 3'→5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.

Highlights

• Active in PCR buffers

Applications

• Primer removal from PCR mixtures:
• prior to PCR product sequencing
• for one-tube 'megaprimer' PCR mutagenesis

• Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
• Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus

Note

The enzyme is not suitable for removing 3'-overhangs of dsDNA.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
20 U/μL
Enzyme
Exonuclease
Quantity
20,000 U
Product Type
Exonuclease I