Thermo Scientific™

Endonuclease IV, E.coli (2 U/µL)

Catalog number:  EN0591

 Related applications:

Genotyping & Genomic Profiling

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Thermo Scientific Endonuclease IV (Endo IV) recognizes apurinic/apyrimidinic (AP) sites of dsDNA and cleaves the phosphodiester bond 5' to the lesion generating a hydroxyl group at the 3'-terminus (see Figure 1 in Supporting Data). The enzyme can also act as a 3'-diesterase that is able to release 3'-phosphoglycolate or 3'-phosphate from the damaged ends of dsDNA. Endo IV possesses also a 3'→5' exonuclease activity. Its progression on substrates is sensitive to ionic strength, metal ions, EDTA, and reducing conditions. Substrates with 3'-recessed ends are preferred substrates for the 3'→5' exonuclease activity.

The enzyme has no requirement for Mg2+ and is fully active in the presence of EDTA in moderate concentrations.


• Studies of DNA damage and repair
• Single cell electrophoresis (comet assay)
• Antitumor drug research
• DNA structure research
• SNP analysis
For Research Use Only. Not for use in diagnostic procedures.


Enzyme: Endonuclease
Product Size: 100 units


Manuals & protocols