DNA Polymerase I (10 U/µL)

Catalog number: EP0042

Thermo Scientific™  Related applications: Nucleic Acid Labeling & Oligo Synthesis | cDNA Libraries & Library Construction

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Thermo Scientific DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity, and ribonuclease H activity.


• Incorporates modified nucleotides (e.g. biotin-, digoxigenin-, aminoallyl-, fluorescently-labeled nucleotides)
• Active in multiple buffers, including restriction enzyme, PCR, and RT buffers


• DNA labeling by nick-translation in conjunction with DNase
• Second-strand synthesis of cDNA in conjunction with RNase H
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: DNA Polymerase I
Product Size: 2,500 units


Manuals & protocols

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