Thermo Scientific™

Klenow Fragment (10 U/µL)

Catalog number: EP0051
Thermo Scientific™

Klenow Fragment (10 U/µL)

Catalog number: EP0051
Catalog Number
EP0051
Unit Size
300 units
Price (USD)
114.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1,500 units
Price (USD)
464.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EP0051300 units
114.00
Your Price:
***
EP00521,500 units
464.00
Your Price:
***
Product Overview
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Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I.

Highlights

• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
• Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers

Applications
• DNA blunting by fill-in 5'-overhangs
• Random-primed DNA labeling
• Labeling by fill-in 5'-overhangs of dsDNA
• DNA sequencing by the Sanger method
• Site-specific mutagenesis of DNA with synthetic oligonucleotides
• Second strand synthesis of cDNA

Specifications

Concentration
10 U/μL
Polymerase
DNA Polymerase I
Quantity
300 U
Product Type
Klenow Fragment

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