Thermo Scientific™

T4 DNA Polymerase (5 U/µL)

Catalog number: EP0061
Thermo Scientific™

T4 DNA Polymerase (5 U/µL)

Catalog number: EP0061
Catalog Number
EP0061
Unit Size
100 units
Price (USD)
Price: 58.25
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
500 units
Price (USD)
Price: 222.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EP0061100 units
Price: 58.25
Your Price:
***
EP0062500 units
Price: 222.00
Your Price:
***
Product Overview
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Thermo Scientific T4 DNA Polymerase is a template-dependent DNA polymerase that catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity.

Highlights

Stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA and greater (more than 200 times) than DNA polymerase I, E. coli,and Klenow fragment
• Active in Thermo Scientific restriction enzyme, PCR, RT and T4 DNA Ligase buffers

Applications

• Blunting of DNA ends: fill-in of 5'-overhangs or/and removal of 3'-overhangs(see​ References1, 2)
• Blunting of PCR products with 3'-dA overhangs
• Synthesis of labeled DNA probes by the replacement reaction(see​ Reference3)
• Oligonucleotide-directed site-specific mutagenesis(see​ Reference4)
• Ligation-independent cloning of PCR products
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase
T4 DNA Polymerase
Quantity
100 unit
Includes
1mL of 5X Reaction Buffer

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