Thermo Scientific™

SP6 RNA Polymerase (20 U/µL)

Catalog number:  EP0131

Related applications:

Nucleic Acid Labeling & Oligo Synthesis

| In Vitro Transcription

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Description

Thermo Scientific Bacteriophage SP6 RNA polymerase is a DNA-dependent RNA polymerase with strict specificity for its respective double-stranded promoters. It catalyzes the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from its promoter and incorporates modified nucleotides.

Highlights

Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Applications

• Synthesis of unlabeled and labeled RNA that can be used:
• For hybridization, in vitro RNA translation
• As aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing
• In studies of RNA secondary structure and RNA-protein interactions, RNA splicing

Consensus promoter sequence:
SP6: ATTTAGGTGACACTATAGAAGNG
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity: 2,000U
Polymerase: SP6 RNA Polymerase