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Thermo Scientific™

Terminal Deoxynucleotidyl Transferase (20 U/µL)

Catalog number: EP0161
Thermo Scientific™

Terminal Deoxynucleotidyl Transferase (20 U/µL)

Catalog number: EP0161
Catalog Number
EP0161
Unit Size
500 units
Price (USD)
Price: 92.50
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
2,500 units
Price (USD)
Price: 360.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EP0161500 units
Price: 92.50
Your Price:
-
EP01622,500 units
Price: 360.00
Your Price:
-
Product Overview
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Additional Information
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Thermo Scientific Terminal Deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase that catalyzes the repetitive addition of deoxyribonucleotides to the 3'-OH of oligodeoxyribonucleotides and single-stranded and double-stranded DNA. TdT requires an oligonucleotide of at least three nucleotides to serve as a primer. With RNA as template TdT shows variable performance which strongly depends upon the tertiary structure of acceptor RNA 3'-end and the nature of nucleotide. Generally, it is lower than using DNA as a template.

Highlights

Incorporates modified nucleotides (e.g., fluorescein-, biotin-, aminoallyl-labeled nucleotides)

Applications

• Production of synthetic homo- and heteropolymers
• Homopolymeric tailing of linear duplex DNA with any type of 3'-OH terminus
• Oligodeoxyribonucleotide and DNA labeling
• 5'-RACE (Rapid Amplification of cDNA Ends)
In situ localization of apoptosis

Note

Due to the presence of CoCl2, the TdT Reaction Buffer is incompatible with downstream applications. It is necessary to remove CoCl2 from the reaction mixture by spin column or phenol/chloroform extraction and subsequent ethanol precipitation.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
20 U/μL
Enzyme
Terminal Deoxynucleotidyl Transferase
Compatible Buffer
Storage Buffer, 5X Reaction Buffer
Quantity
500 U
Product Type
TdT