Informational:For PCR enzyme with ≤0.01 copy of bacterial gDNA per enzyme unit, check out Invitrogen Platinum Taq DNA Polymerase, DNA-free.
Thermo Scientific™

Taq DNA Polymerase, native, without BSA (5 U/µL)

Catalog number: EP0282
Thermo Scientific™

Taq DNA Polymerase, native, without BSA (5 U/µL)

Catalog number: EP0282
Catalog Number
EP0282
Unit Size
500 units
Price (USD)
Price: 229.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EP0282500 units
Price: 229.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E. coli.

Highlights

Thermostable—half life is more than 40 min at 95°C.
Generates PCR products with 3'-dA overhangs.
Supplied with two buffers—10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.
Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).

Applications

• Routine PCR amplification of DNA fragments up to 5 kb
• DNA labeling (see Reference s 1-3)
• High throughput PCR
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
PCR Amplification
Concentration
10X
Detection Method
Primer-Probe
Fidelity (vs. Taq)
1 X
GC-Rich PCR Performance
Low
Hot Start
No
Overhang
3'-A
Polymerase
Taq DNA Polymerase
Reaction Format
Standalone
Reaction Speed
Standard
Starting Material
DNA
Volume
2 mL
Includes
2 x 1 mL of 25 mM MgCl2, 2 x 1 mL of 10X Taq Buffer with KCl, 2 x 1 mL of 10X Taq Buffer with (NH4)2SO4
Product Type
Taq DNA Polymerase

Contents & Storage

Contains:
• 100 µL Taq DNA Polymerase, native (5 U/µL)
• 2 x 1.25 mL 10X Taq Buffer with KCl
• 2 x 1.25 mL 10X Taq Buffer with (NH4)2SO4
• 2 x 1.25 mL 25 mM MgCl2

Store at -20°C.

Figures