Thermo Scientific Klenow Fragment, exo-, is the large fragment of DNA polymerase I . It exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities of DNA Polymerase I. The 3'→5' exonuclease activity of the enzyme is eliminated by mutations in the 3'→5'-exonuclease active site.
• Lacks 3'→5' exonuclease activity • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, fluorescein-, rhodamine-, aminoallyl-, biotin-labeled nucleotides) • Active in restriction enzyme, PCR, and RT buffers
Applications • Random-primed DNA labeling • Labeling by fill-in 5'-overhangs of dsDNA • Strand displacement amplification (SDA) • DNA sequencing by the Sanger method
Klenow Fragment, exo- is not recommended for DNA blunting reactions prior to DNA ligation since it frequently adds one or more extra nucleotides to the 3'-terminus of blunt-end DNA substrates in a non-template directed fashion.
For Research Use Only. Not for use in diagnostic procedures.