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Thermo Scientific™

RevertAid Reverse Transcriptase (200 U/μL)

Catalog number: EP0441
Thermo Scientific™

RevertAid Reverse Transcriptase (200 U/μL)

Catalog number: EP0441
Catalog Number
EP0441
Unit Size
10,000 units
Price (USD)
Price: 108.00
Online price: 72.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
5 x 10,000 units
Price (USD)
Price: 448.00
Online price: 340.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EP044110,000 units
Price: 108.00
Online price: 72.65
Your price:
-
EP04425 x 10,000 units
Price: 448.00
Online price: 340.65
Your price:
-
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Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids that is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Features of RevertAid Reverse Transcriptase include:
• Efficient synthesis of full-length first strand cDNA up to 13 kb
• Optimum activity at 42°C
• Active up to 50°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)

Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension

Source
RevertAid Reverse Transcriptase is sourced from E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Stand-alone Enzyme
Reaction Speed
60 min.
Technique
Reverse Transcription
Optimal Reaction Temperature
42°C
Quantity
10,000 Units
Reverse Transcriptase
RevertAid
Ribonuclease H Activity
Yes
Shipping Condition
Dry Ice
Concentration
200 U/μL
Final Product Type
First-Strand cDNA
Reaction Format
Separate components
Reagent Type
Reverse Transcription
Size (Final Product)
Up to 13 kb
Starting Material
RNA

Contents & Storage

• RevertAid Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.

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