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Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids that is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Features of RevertAid Reverse Transcriptase include:
• Efficient synthesis of full-length first strand cDNA up to 13 kb
• Optimum activity at 42°C
• Active up to 50°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension
Source
RevertAid Reverse Transcriptase is sourced from E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.
Format | Stand-alone Enzyme |
Reaction Speed | 60 min. |
Technique | Reverse Transcription |
Optimal Reaction Temperature | 42°C |
Quantity | 10,000 Units |
Reverse Transcriptase | RevertAid |
Ribonuclease H Activity | Yes |
Shipping Condition | Dry Ice |
Concentration | 200 U/μL |
Final Product Type | First-Strand cDNA |
Reaction Format | Separate components |
Reagent Type | Reverse Transcription |
Size (Final Product) | Up to 13 kb |
Starting Material | RNA |
Unit Size | 10,000 units |
Format | Stand-alone Enzyme |
Reaction Speed | 60 min. |
Technique | Reverse Transcription |
Optimal Reaction Temperature | 42°C |
Quantity | 5 x 10,000 Units |
Reverse Transcriptase | RevertAid |
Ribonuclease H Activity | Yes |
Shipping Condition | Dry Ice |
Concentration | 200 U/μL |
Final Product Type | First-Strand cDNA |
Reaction Format | Separate components |
Reagent Type | Reverse Transcription |
Size (Final Product) | Up to 13 kb |
Starting Material | RNA |
Unit Size | 5 x 10,000 units |
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EP0441 | 10,000 units | 10,000 Units | Price: 108.00 Online price: 72.65 Your price: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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EP0442 | 5 x 10,000 units | 5 x 10,000 Units | Price: 448.00 Online price: 340.65 Your price: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids that is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Features of RevertAid Reverse Transcriptase include:
• Efficient synthesis of full-length first strand cDNA up to 13 kb
• Optimum activity at 42°C
• Active up to 50°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension
Source
RevertAid Reverse Transcriptase is sourced from E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.
General References:
Verma, I.M., Reverse transcriptase, The Enzymes (Boyer, P.D., ed), Academic Press Inc., vol. 14, 87–103, 1981., Gerard, G.F., D'Alessio, J.M., Methods in Molecular Biology, 16, Humana Press, Totowa, N.J., 73-
• RevertAid Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer
Store at –20°C.