Thermo Scientific™

RevertAid Reverse Transcriptase (200 U/µL)

Catalog number:  EP0441

Related applications:

PCR & Real-Time PCR

| Cloning

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Description

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Highlights

• Efficient synthesis of full-length first strand cDNA up to 13 kb
• Optimum activity at 42°C
• Active up to 50°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)

Applications

• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Optimal Reaction Temperature: 42° C
Product Line: RevertAid
Reverse Transcriptase: RevertAid
Ribonuclease H Activity: Yes
Size (Final Product): 13 kb or less
Includes: 1 x 1mL of 5X Reaction Buffer

Contents & storage

RevertAid Reverse Transcriptase (200 U/μL) is supplied with 5X Reaction Buffer (250 mM Tris-HCl (pH 8.3 at 25°C), 250 mM KCl, 20 mM MgCl2, 50 mM DTT).
Store at -20°C.