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Thermo Scientific™

RevertAid Reverse Transcriptase (200 U/µL)

Catalog number: EP0441
Thermo Scientific™

RevertAid Reverse Transcriptase (200 U/µL)

Catalog number: EP0441
Catalog Number
EP0441
Unit Size
10,000 units
Price (USD)
Price: 104.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
5 x 10,000 units
Price (USD)
Price: 432.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EP044110,000 units
Price: 104.00
Your Price:
-
EP04425 x 10,000 units
Price: 432.00
Your Price:
-
Product Overview
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Additional Information
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Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Highlights

• Efficient synthesis of full-length first strand cDNA up to 13 kb
• Optimum activity at 42°C
• Active up to 50°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)

Applications

• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
200 U/μL
Final Product Type
First-Strand cDNA
Format
Reverse Transcriptase
Reaction Format
Separate components
Reaction Speed
60 min.
Reagent Type
Reverse Transcription
Size (Final Product)
Up to 13 kb
Starting Material
RNA
Optimal Reaction Temperature
42°C
Quantity
10,000 Units
Reverse Transcriptase
RevertAid
Ribonuclease H Activity
Yes

Contents & Storage

• RevertAid Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.