Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.
• Efficient synthesis of full-length first strand cDNA up to 13 kb • Optimum activity at 42°C • Active up to 50°C • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR • Synthesis of cDNA for cloning and expression • Generation of labeled cDNA probes for microarrays • DNA labeling • Analysis of RNA by primer extension
For Research Use Only. Not for use in diagnostic procedures.
Optimal Reaction Temperature:
Ribonuclease H Activity:
Size (Final Product):
13 kb or less
1 x 1mL of 5X Reaction Buffer
Contents & storage
RevertAid Reverse Transcriptase (200 U/μL) is supplied with 5X Reaction Buffer (250 mM Tris-HCl (pH 8.3 at 25°C), 250 mM KCl, 20 mM MgCl2, 50 mM DTT). Store at -20°C.