RevertAid Reverse Transcriptase (200 U/μL)
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Thermo Scientific™
RevertAid Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structureRead more
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Catalog NumberQuantity
EP044110,000 Units
EP04425 x 10,000 Units
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Catalog number EP0441
Price (USD)/ 10,000 units
110.00
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10,000 Units
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Price (USD)/ 10,000 units
110.00
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RevertAid Reverse Transcriptase (200 U/μL)
Catalog numberEP0441
Price (USD)/ 10,000 units
110.00
-
Add to cart

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids that is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Features of RevertAid Reverse Transcriptase include:
• Efficient synthesis of full-length first strand cDNA up to 13 kb
• Optimum activity at 42°C
• Active up to 50°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)

Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension

Source
RevertAid Reverse Transcriptase is sourced from E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatStand-alone Enzyme
Reaction Speed60 min.
TechniqueReverse Transcription
Optimal Reaction Temperature42°C
Quantity10,000 Units
Reverse TranscriptaseRevertAid
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
Concentration200 U/μL
Final Product TypeFirst-Strand cDNA
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 13 kb
Starting MaterialRNA
Unit Size10,000 units
Contents & Storage

• RevertAid Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.