Thermo Scientific™

RevertAid H Minus Reverse Transcriptase (200 U/µL)

Catalog number: EP0451
Thermo Scientific™

RevertAid H Minus Reverse Transcriptase (200 U/µL)

Catalog number: EP0451
Catalog Number
EP0451
Unit Size
10,000 units
Price (USD)
Price: 135.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5 x 10,000 units
Price (USD)
Price: 550.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EP045110,000 units
Price: 135.00
Your Price:
***
EP04525 x 10,000 units
Price: 550.00
Your Price:
***
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Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).

Highlights

• High yields of full-length first strand cDNA up to 13 kb
• Optimum activity at 42 to 45°C
• Active up to 55°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)

Applications

• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension

Related Products
RevertAid H Minus Reverse Transcriptase (200 U/µL)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Size (Final Product)
13 kb or Less
Includes
1mL 5X Reaction Buffer
Optimal Reaction Temperature
42°C to 45°C
Product Line
RevertAid
Quantity
10,000 units
Reverse Transcriptase
RevertAid H Minus
Ribonuclease H Activity
None

Contents & Storage

RevertAid H Minus Reverse Transcriptase (200 U/μL) is supplied with 5X Reaction Buffer (250 mM Tris-HCl (pH 8.3 at 25°C), 250 mM KCl, 20 mM MgCl2, 50 mM DTT).
Store at -20°C.

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