Thermo Scientific™

Bsm DNA Polymerase, large fragment (8 U/µL)

Catalog number:  EP0691

 Related applications:

Nucleic Acid Labeling & Oligo Synthesis

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Description

Thermo Scientific Bsm DNA Polymerase, Large Fragment, is an equivalent to Bst DNA polymerase, which catalyzes 5'→3' synthesis of DNA and lacks 5'→3' and 3'→5' exonuclease activities. Bsm DNA Polymerase, Large Fragment, is a portion of DNA polymerase of Bacillus smithii that has a strong strand displacement activity and is active in a wide range of temperatures from 30°C to 63°C, with an optimum of activity at 60°C. It is an enzyme with high functional similarity to Bst DNA Polymerase, Large Fragment, and can replace it in most applications.

Highlights
• Thermophilic DNA polymerase with strong strand displacement activity

Applications
• Isothermal DNA amplification by the method of:
    --Loop-mediated isothermal amplification (LAMP)
    --Whole genome amplification (WGA)
    --Ramification amplification (RAM)
• Random-primed DNA labeling
• Labeling by fill-in 5'-overhangs of dsDNA

Note: Not suitable for use in PCR.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: Bsm DNA Polymerase
Product Size: 1,600 units

Contents & storage

• 200 µL Bsm DNA Polymerase, Large Fragment (8 U/µL)
• 1.25 mL 10X Bsm Buffer

Store at -20°C.

Documents

Manuals & protocols