Thermo Scientific Bsm DNA Polymerase, Large Fragment, is an equivalent to Bst DNA polymerase, which catalyzes 5'→3' synthesis of DNA and lacks 5'→3' and 3'→5' exonuclease activities. Bsm DNA Polymerase, Large Fragment, is a portion of DNA polymerase of Bacillus smithii that has a strong strand displacement activity and is active in a wide range of temperatures from 30°C to 63°C, with an optimum of activity at 60°C. It is an enzyme with high functional similarity to Bst DNA Polymerase, Large Fragment, and can replace it in most applications.
Highlights • Thermophilic DNA polymerase with strong strand displacement activity
Applications • Isothermal DNA amplification by the method of: --Loop-mediated isothermal amplification (LAMP) --Whole genome amplification (WGA) --Ramification amplification (RAM) • Random-primed DNA labeling • Labeling by fill-in 5'-overhangs of dsDNA
Note: Not suitable for use in PCR.
For Research Use Only. Not for use in diagnostic procedures.
Bsm DNA Polymerase
Contents & storage
• 200 µL Bsm DNA Polymerase, Large Fragment (8 U/µL) • 1.25 mL 10X Bsm Buffer