Informational:Maxima H Minus cDNA Synthesis Master Mix now available. Get a sample »
Thermo Scientific™

Maxima Reverse Transcriptase (200 U/µL)

Catalog number: EP0741
Thermo Scientific™

Maxima Reverse Transcriptase (200 U/µL)

Catalog number: EP0741
Catalog Number
EP0741
Unit Size
2,000 units
Price (USD)
Price: 72.50
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
10,000 units
Price (USD)
Price: 229.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
4 x 10,000 units
Price (USD)
Price: 744.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EP07412,000 units
Price: 72.50
Your Price:
***
EP074210,000 units
Price: 229.00
Your Price:
***
EP07434 x 10,000 units
Price: 744.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity. The engineered enzyme features dramatically improved thermostability and robustness, and increased synthesis rates compared to wild type M-MuLV RT.

Maxima Reverse Transcriptase is capable of reproducible cDNA synthesis from a wide range of input total RNA amounts (from 1 pg to 5 µg) at elevated temperatures (50 to 65°C), which makes this enzyme an ideal tool for two step RT-qPCR (see supporting data).

Due to its high thermostability, Maxima enzyme maintains full activity during the entire reverse transcription reaction, generates the highest yields of cDNA, and is able to synthesize even very long RNA transcripts up to 20 kb. The reaction temperature can be increased up to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. Due to its increased synthesis rate, the reverse transcriptase reaction can be completed in 15 to 30 min.

Highlights

• High yields of full-length cDNA up to 20 kb
• Active up to 65°C
• Thermostable—90% active after incubation at 50°C for 60 minutes
• High sensitivity—reproducible cDNA synthesis from a wide range of total RNA quantities (1 pg to 5 µg)
• Efficient—complete cDNA synthesis in 15 to 30 minutes
• Incorporates modified nucleotides

Applications

• Two step RT-PCR
• Two step RT-qPCR
• First strand cDNA synthesis
• Construction of full length cDNA libraries
• DNA labeling
• Primer extension

Also available: Maxima First Strand cDNA Synthesis Kit for RT-qPCR
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Size (Final Product)
20 kb or Less
Optimal Reaction Temperature
50°C to 55°C
Product Line
Maxima™
Quantity
2000 unit
Reverse Transcriptase
Maxima
Ribonuclease H Activity
Yes

Contents & Storage

Maxima Reverse Transcriptase (provided as 2000 Units at 200 U/μL concentration) is supplied with 5X RT Buffer (250 mM Tris-HCl (pH 8.3 at 25°C), 375 mM KCl, 15 mM MgCl2, 50 mM DTT).
Store at -20°C.

Figures