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Thermo Scientific™

Maxima H Minus Reverse Transcriptase (200 U/μL)

Catalog number: EP0752
Thermo Scientific™

Maxima H Minus Reverse Transcriptase (200 U/μL)

Catalog number: EP0752
Catalog Number
EP0752
Unit Size
10,000 units
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
2,000 units
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
4 x 10,000 units
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EP075210,000 units
-
EP07512,000 units
-
EP07534 x 10,000 units
-
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Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT. The engineered enzyme features dramatically improved thermostability, 50X higher processivity, robustness, and increased synthesis rate compared to wild type M-MuLV RT.

The eliminated RNase H activity enables the enzyme to produce very long RNA transcripts up to 20 kb. Due to its high thermostability, the enzyme maintains full activity during the entire reverse transcription reaction and generates high yields of cDNA. The reaction temperature can be increased to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. The extremely high processivity of Maxima H Minus enzyme results in increased resistance to common reaction inhibitors, such as guanidine, formamide, and ethanol. 

Features of Maxima H Minus Reverse Transcriptase include:
• Thermostable—90% active after incubation at 50°C for 60 minutes in a reaction mixture
• Active up to 65°C
• High yields of full-length cDNA up to 20 kb
• High sensitivity—reproducible cDNA synthesis from a wide range of starting total RNA amounts (1 pg to 5 μg)
• Efficient—complete cDNA synthesis in 15 to 30 minutes
• Increased resistance to common reaction inhibitors
• Incorporates modified nucleotides

Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• Analysis of RNA by primer extension

Related products
Maxima H Minus Reverse Transcriptase
Maxima H Minus First Strand cDNA Synthesis Kit
Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase
Maxima H Minus cDNA Synthesis Master Mix
Maxima H Minus cDNA Synthesis Master Mix, with dsDNase

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Stand-alone Enzyme
GC-Rich PCR Performance
High
Reaction Speed
15 to 30 min.
Technique
Reverse Transcription
Optimal Reaction Temperature
50°C to 55°C
Quantity
10,000 Units
Reverse Transcriptase
Maxima H Minus
Ribonuclease H Activity
Reduced
Shipping Condition
Dry Ice
Concentration
200 U/μL
Final Product Type
First-Strand cDNA
Reaction Format
Separate components
Reagent Type
Reverse Transcription
Size (Final Product)
Up to 20 kb
Starting Material
RNA

Contents & Storage

• Maxima H Minus Reverse Transcriptase, 1 x 10,000 units (200 U/μL)
• 5X RT Buffer

Store at –20°C.

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