E-PAGE™ Midi Protein Gels, 3.7 mm
E-PAGE™ Midi Protein Gels, 3.7 mm
Citations & References (1)
Invitrogen™

E-PAGE™ Midi Protein Gels, 3.7 mm

E-PAGE™ Gels are self-contained, pre-cast gels that include a gel matrix and electrodes packaged inside a disposable cassette.
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Catalog NumberWellsGel Percentage
EP0960696-well6%
EP0480848-well8%
Catalog number EP09606
Price (MXN)
-
Wells:
96-well
Gel Percentage:
6%
E-PAGE Gels are self-contained, pre-cast gels that include a gel matrix and electrodes packaged inside a disposable cassette. Protein separation on an E-PAGE Gel requires an EBase Integrated Device, a compact and automated platform designed for medium- and high-throughput electrophoresis.

Each E-PAGE 8% 48-well gel contains 48 sample lanes and 4 marker lanes with a 3.2-cm separation length. E-PAGE 48-well gels can be loaded with a 12- or 8-channel pipettor or 4- or 8-tip robotic system (Figure 1).

Each E-PAGE 6% 96-well gel contains 96 sample lanes and 8 marker lanes with a 1.6-cm separation length. The well openings of the cassette are compatible with the standard 96-well plate format and can be conveniently loaded with a multichannel pipettor or 8-, 12-, or 96-tip liquid-handling robotic device (Figure 2).

Both E-PAGE 48 and 96 cassettes can be opened to remove the gel for downstream staining or blotting (Figures 3 and 4). In addition, each cassette is labeled with an individual barcode to facilitate identification of the gel using commercial barcode readers.

More about the E-PAGE precast gel system
The Invitrogen E-PAGE precast gel system is designed for fast, mess-free medium- and high-throughput protein electrophoresis. It consists of the E-PAGE precast gels, E-Base Integrated devices for running the gels, and free E-Editor 2.0 software for image analysis.

The all-in-one E-Gel Power Snap Plus Electrophoresis System is designed for fast and convenient high-throughput gel electrophoresis. With dry precast E-PAGE gel technology, you can run protein samples in as little as 14 minutes. The device arrives with pre-programmed protocols for both E-PAGE gel types.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Gel Thickness3.7 mm
Length (Metric)8 cm
Mode of SeparationMolecular Weight
Product LineE-PAGE
Quantity8 gels (1 kit)
Sample Loading VolumeUp to 20 μL
Shelf Life6 Months
Shipping ConditionRoom Temperature
Storage RequirementsStore at room temperature, do not allow the temperature to drop below 4°C or rise above 40°C
Width (Metric)13 cm
For Use With (Equipment)E-BASE
Gel Percentage6%
Gel SizeMidi
Gel TypeE-PAGE
Separation Range10 to 300 kDa
Separation TypeDenaturing
Wells96-well
Unit SizeEach

Frequently asked questions (FAQs)

Are there robotic scripts for running E-Gel 48/96 or E-PAGE 48/96 gels?

Robotic scripts for running E-Gel 96 Agarose Gels and E-PAGE 96 Gels on Beckman Coulter's Biomek FX workstation can be found on our website at: www.thermofisher.com/egels (click the Labware Definitions link in the left navigation pane).

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the difference between the EG and EP program for the E-Base system?

The EG program is to run E-Gel 96 and 48 gels, while the EP is to run the E-PAGE 96 and 48 gels.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What sample loading buffer should be used for E-PAGE gels?

For SDS-PAGE and staining or blotting, we recommend using the 4X E-PAGE Loading Buffer 1 for preparing samples. We do not recommend using any other SDS-PAGE sample buffer. The E-PAGE Loading Buffer 1 is optimized for E-PAGE gels. If the sample is already in NuPAGE LDS sample buffer or the Tris-Glycine SDS sample buffer, it is fine to run on the E-PAGE gels. However, there may be a loss of resolution in the bands.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Where can I download the latest version of the E-Gel 96 analysis software?

The E-Editor 2.0 Software can be downloaded for free from the Thermo Fisher Scientific website (search for 'e-editor 2.0').

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Are the E-PAGE Gels (example Cat. No. EP09606) compatible with the iBlot3 device (Cat. O. IB31001)?

Due to the thickness of the E-PAGE gels, they are not compatible with the iBlot3 device for transfer. Recommended methods for transfer of E-PAGE gels are semi-dry blotting or semi-wet blotting in the XCell II Blot Module. Methods are described in the E-PAGE Technical Guide.

Find additional tips, troubleshooting help, and resources within our Protein Gel Electrophoresis Chambers, Power Supplies, and Accessories Support Center.

View all E-PAGE™ Midi Protein Gels, 3.7 mm FAQs

Citations & References (1)

Citations & References
Abstract
Detection of tetracysteine-tagged proteins using a biarsenical fluorescein derivative through dry microplate array gel electrophoresis.
Authors:Feldman G, Bogoev R, Shevirov J, Sartiel A, Margalit I,
Journal:Electrophoresis
PubMed ID:15300761
The design of an extended-run 96-well sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system and the development of protein detection technology based upon fluorescein derivatives that bind to peptide epitope tags, allows the creation of a truly high-throughput analysis of protein expression, where less than 20 min are needed to separate ... More