Invitrogen™

VEGF-A Human ProcartaPlex™ Simplex Kit

Catalog number:  EPX01A-10277-901

Related applications:

Protein Assays and Analysis

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Description

The Human VEGF-A Simplex ProcartaPlex Kit measures VEGF-A protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample

Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g. Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Range: As determined for Lot 1: 5.86 - 24,000 pg/mL
Gene ID (Entrez): 7422
Sample Volume: Serum, Plasma: 25 µL; CCS: 50 µL
Interassay CV: 3.4%
Intraassay CV: 5.6%
Quantity: 96 tests
Target Species: Human
Sample Type: Serum, Plasma, Cell Culture Supernatants (CCS)
Assay Sensitivity: As determined for Lot 1: 1.73 pg/mL
Bead Region: VEGF-A [78]
Gene Symbol: VEGFA
Gene Alias: MVCD1, VEGF, VPF
Gene: vascular endothelial growth factor A
Protein Aliases: VEGF-A, Vascular permeability factor, VPF
Protein: Vascular endothelial growth factor A
Shipping Condition: Wet Ice
Product Line: ProcartaPlex™
UniProt ID: P15692
Immunoassay Kit Format: Simplex Kit
For Use With (Equipment): Luminex™ Instruments

Contents & storage

• 1 vial Capture Beads (50X)
• 1 vial biotinylated Detection Antibody (50X)
• 2 vials Human Standard Mix C (lyophilized)

Store at 2–8°C.