Invitrogen™

IFN-alpha Mouse ProcartaPlex™ Simplex Kit

Catalog number:  EPX01A-26027-901

 Related applications:

Protein Assays and Analysis

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Description

The Mouse IFN-alpha Simplex ProcartaPlex kit measures IFN-alpha protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Mouse

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Range: As determined for Lot 1: 5.6-22800 pg/mL
Assay Sensitivity: As determined for Lot 1: 3.03 pg/mL
Bead Region: 30
Former Identifiers: eBioscience
Gene Name: interferon alpha 1
Gene Symbol: IFNA1
Immunoassay Kit Format: Simplex Kit
Interassay CV: 5.1%
Intraassay CV: 4.1%
Protein Aliases: IFN-alpha-1, Interferon alpha family gene 1
Protein Name: Interferon alpha-1
Sample Volume: CCS: 50 µL, Serum, Plasma: 25 µL
Target (Kit Named): IFN alpha
Target List: IFN alpha
Target Species (Named): Mouse
Target Species (Validated): Mouse
UniProt ID: P01572
Detection Method: Fluorescent
For Use With (Equipment): Luminex™ Instruments
Gene Aliases: Ifa1
Gene ID: 15962
Label or Dye: R-PE
Product Line: ProcartaPlex™
Product Size: 96 tests
Sample Type (Specific): Cell Culture Supernatants, Plasma, Serum
System: Luminex™

Contents & storage

1 vial concentrated Magnetic Beads coated with monoclonal antibody to mouse IFN alpha
2 vials mouse IFN alpha Standard (lyophilized)
1 vial mouse Biotin-Conjugate (50x) anti-mouse IFN alpha monoclonal antibody

Store at 2-8°C.

Documents

Manuals & protocols