Brain Injury 5-plex Human ProcartaPlex™ Panel

Catalog number: EPX050-15838-901

Brain Injury 5-plex Human ProcartaPlex™ Panel

Catalog number: EPX050-15838-901
Catalog Number
Unit Size
96 tests
Price (USD)
Price: 1,426.00
Your Price:
Catalog NumberUnit SizePrice (USD)Quantity
EPX050-15838-90196 tests
Price: 1,426.00
Your Price:
Product Overview
Citations & References
Additional Information
The Human Brain Injury 5-Plex ProcartaPlex Panel helps enable neurological research by analyzing the protein targets GFAP, NF-H, S100B, NSE, and UCHL1 in a single well using Luminex xMAP technology. Simultaneous detection of these targets, released due to astroglial injury (GFAP, S100B), axonal injury (NF-H), and neuronal cell body injury (UCH-L1), can provide a better understanding of the progression of neurological disorders.

The panel is provided in a ready-to-use format with individual vials of capture and detection reagents formulated at 1X concentration, requiring less pipetting and experimental setup. It is not combinable with simplexes or other panels. It complements the Neuroinflammation 6-Plex ProcartaPlex Panel (Cat. No. EPX060-15833-901) and Neurodegeneration 9-plex ProcartaPlex Panel (Cat. No. EPX090-15836-901), providing 15 additional protein targets in the same research area. ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision.

Target list [bead region]: GFAP [22], NF-H [33], NSE [12], S100B [18], UCHL1 [19]

About ProcartaPlex assays for the Luminex platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Features include:
• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
• Well-established Luminex technology highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

For Research Use Only. Not for use in diagnostic procedures.


Product Type
Multiplex Panel
96 Tests
Bead Type
Bead Region
NSE [12], GFAP [22], pNF-H [33], UCHL-1 [19], S100b [18]
Assay Sensitivity
See Certificate of Analysis
Assay Range
See Certificate of Analysis
Multiplex Kit
For Use With (Equipment)
Luminex Instruments
Interassay CV
Less than 15%
Intraassay CV
Less than 15%
Product Line
Sample Type
Serum, Plasma, Cerebrospinal Fluid
Sample Volume
Serum, Plasma, CSF: 25 µL
Shipping Condition
Wet Ice

Contents & Storage

• 2 vials Human Standard Mix 4 (lyophilized)
• 1 vial Capture Bead Mix (1X)
• 1 vial Biotinylated Detection Antibody Mix (1X)
• 1 bottle Reading Buffer (1X)
• 1 bottle Streptavidin-PE (1X)
• 1 bottle Universal Assay Buffer (1X)
• 1 bottle Wash Buffer (10X)
• 8-tube strip
• Adhesive film
• Flat-bottom 96-well plate, black

Store at 2–8°C.


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