Invitrogen™

ProcartaPlex™ Human Atherosclerosis Panel 1, 9plex

Catalog number: EPX090-15822-901
Have Questions?
Invitrogen™

ProcartaPlex™ Human Atherosclerosis Panel 1, 9plex

Catalog number: EPX090-15822-901
Have Questions?
Catalog Number
EPX090-15822-901
Unit Size
96 tests
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EPX090-15822-90196 tests
-
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The ProcartaPlex Human Atherosclerosis Panel 1 9plex enables cardiovascular research by analyzing 9 protein targets in a single well using Luminex xMAP technology. Simultaneous detection of these targets can help explore disease areas such as coronary artery disease, stroke, peripheral artery disease, or kidney problems. The panel is provided in a ready-to-use format with individual vials of capture and detection reagents formulated at 1X concentration, requiring less pipetting and experimental setup. It is not combinable with simplexes or other panels. It complements the Human Atherosclerosis Panel 2 4plex (Cat. No. EPX040-15824-901) and Human Arteriosclerosis Panel 5plex (Cat. No. EPX050-15821-901) that provides 9 additional protein targets in the same research area.

ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit our ProcartaPlex Immunoassays page for more information and available products.

Target list [bead region]: FGF-2 [75], HSP60 [13], IL-6 [25], IL-8 (CXCL8) [27], LOX-1 [76], Myeloperoxidase (MPO) [18], PAI-1 (Serpin) [35], Tenascin-C [28], TRAIL [66]

About ProcartaPlex assays for the Luminex platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.

  • Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
  • More results per sample—measure multiple protein targets simultaneously in a single 25–50 μL sample
  • Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Multiplex Panel
Quantity
96 Tests
Species
Human
Combinability
Non-Combinable
Assay Sensitivity
See Certificate of Analysis
Assay Range
See Certificate of Analysis
Format
Multiplex Kit
For Use With (Equipment)
Luminex™ Instruments
Product Line
ProcartaPlex™
Sample Type
Serum, Plasma, Cell Culture Supernatants
Sample Volume
Serum, Plasma: 25 μL, CCS: 50 μL
Shipping Condition
Wet Ice
Research Area
Cardiovascular

Contents & Storage

• 2 vials Human Standard Mix U1 (lyophilized)
• 2 vials Human Standard Mix U2 (lyophilized)
• 1 vial Capture Bead Mix (1X)
• 1 vial Biotinylated Detection Antibody Mix (1X)
• 1 bottle Reading Buffer (1X)
• 1 bottle Wash Buffer (10X)
• 1 bottle Streptavidin-PE (1X)
• 1 bottle Universal Assay Buffer (1X)
• 8-tube strip
• Adhesive film
• Greiner Microplate, black, 96-well

Store at 2°C to 8°C

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