Invitrogen™

Apoptotic Cell Clearance 12-plex Human ProcartaPlex™ Panel

Catalog number:  EPX120-15816-901

Related applications:

Protein Assays and Analysis

Back to top

Description

The Human Apoptotic Cell Clearance 12-plex ProcartaPlex Panel enables the study of immune function by analyzing 12 protein targets in a single well using Luminex xMAP technology. This panel allows simultaneous detection of soluble receptors and molecules involved in recognition and removal of an apoptotic cell by a neighboring cell or a phagocyte. Apoptotic cell clearance is typically anti-inflammatory and immunologically silent, however defects in apoptotic cell removal can be linked with autoimmunity and inflammation. The panel is provided in a ready-to-use format with individual vials of capture and detection reagents formulated at 1X concentration, requiring less pipetting and experimental setup. It is not combinable with simplexes or other panels.

ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.

Target list [bead region]: AXL [27], CD36 [38], CALR (CRT) [73], Gas6 [53], LOX-1 [76], MBL [55], Mer (MERTK) [26], CD31 (PECAM-1) [72], PAI-1 (Serpin) [35], uPAR [61], RAGE [47], TYRO3 [37]

About ProcartaPlex assays for the Luminex platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.

Features include:
• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 μL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Range: See Certificate of Analysis
Sample Volume: Serum, Plasma: 25 µL; CCS: 50 µL
Interassay CV: Less than 15%
Intraassay CV: Less than 15%
Quantity: 96 tests
Target Species: Human
Sample Type: Serum, Plasma, Cell Culture Supernatants
Multiplex Size Plex No.: 12
Shipping Condition: Wet Ice
Product Line: ProcartaPlex™
Immunoassay Kit Format: Multiplex Kit
For Use With (Equipment): Luminex™ Instruments

Contents & storage

• 2 vials Human Standard Mix S1 (lyophilized)
• 2 vials Human Standard Mix S2 (lyophilized)
• 1 vial Capture Bead Mix (1 X)
• 1 vial Biotinylated Detection Antibody Mix (1 X)
• 1 Bottle Reading Buffer (1 X)
• 1 Bottle Wash Buffer (10 x)
• 1 Bottle Streptavidin-PE (1 X)
• 1 Bottle Universal Assay Buffer (1 X)
• 8-tube strip
• Adhesive film
• Flat-bottom 96-well plate, black

Store (at 2°C to 8°C.)