Invitrogen™

TNF Superfamily 15-Plex Human ProcartaPlex™ Panel

Catalog number:  EPX150-15801-901

 Related applications:

Protein Assays and Analysis

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Description

The Human TNF Superfamily 15-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 15 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
4-1BBL; APRIL; BAFF; CD30L; EDA-1; FasL; GITRL; CD40L; NGF beta; TNF alpha; TNF beta; LIGHT; RANKL; TRAIL; TWEAK

Bead Regions
See product documentation

Assay Range
[As determined for Lot 1:] 4-1BBL: 168.95-692000 pg/mL; APRIL:72.53-297100 pg/mL; BAFF: 2.64-10800 pg/mL; CD30L: 185.1-758300 pg/mL; EDA-1: 28.25-28925 pg/mL; FasL: 2.2-9000 pg/mL; GITRL: 3.31-13575 pg/mL; CD40L: 5.15-21100 pg/mL; NGF beta: 8.47-34700 pg/mL; TNF alpha: 6.86-28100 pg/mL; TNF beta: 2.47-10125 pg/mL; LIGHT: 1.27-5200 pg/mL; RANKL: 7.71-31600 pg/mL; TRAIL: 1.99-8150 pg/mL; TWEAK: 37.6-154000 pg/mL

Assay Sensitivity
See product documentation

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Sensitivity: See product documentation
Bead Region: See product documentation
Detection Method: Fluorescent
For Use With (Equipment): Luminex™ Instruments
Former Identifiers: eBioscience
Immunoassay Kit Format: Panel
Interassay CV: Less than 10%
Intraassay CV: Less than 10%
Label or Dye: R-PE
Multiplex Panel Name: TNF Superfamily
Multiplex Size (Plex No.): 15
Product Line: ProcartaPlex™
Product Size: 96 tests
Sample Type (Specific): Cell Culture Supernatants, Plasma, Serum
Sample Volume: CCS: 50 µL, Serum, Plasma: 25 µL
System: Luminex™
Target Species (Named): Human
Target Species (Validated): Human

Contents & storage

2 vials Human Standard Mix I
2 vials Human Standard Mix K
1 vial Human TNF Superfamily Bead Mix
1 vial Human TNF Superfamily Detection Antibody Mix
1 bottle Reading Buffer
1 bottle Wash Buffer 10x
1 bottle Streptavidin-PE
1 bottle Universal Assay Buffer
1 bottle Detection Antibody Diluent
PCR 8-Tube Strip
Adhesive Film
Greiner Microplate black 96 well

Store at 2-8°C.

Documents

Manuals & protocols