Thermo Scientific™

Cfr42I (SacII) (10 U/µL)

Catalog number: ER0201
Thermo Scientific™

Cfr42I (SacII) (10 U/µL)

Catalog number: ER0201
Catalog Number
ER0201
Unit Size
1200 units
Price (USD)
47.55
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5 x 1200 units
Price (USD)
176.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
2,000 units
Price (USD)
74.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
ER02011200 units
47.55
Your Price:
***
ER02025 x 1200 units
176.00
Your Price:
***
ER02052,000 units
74.00
Your Price:
***
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Thermo Scientific Cfr42I (SacII) restriction enzyme recognizes CCGC^GG sites and cuts best at 37°C in B buffer (Isoschizomers: KspI, SacII, Sfr303I, SgrBI, SstII). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: Cfr42I activity is affected by high salt concentration. Trace amounts of sodium chloride remaining in the substrate DNA after completion of upstream applications may inhibit enzyme activity and result in impaired DNA cleavage. Certain sites in lambda DNA are difficult to cleave with Cfr42I, the same as with its prototype SacII. At least two copies of Cfr42I recognition site are required for efficient cleavage.
For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Methylation Sensitivity
Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive
Enzyme
Cfr42I (SacII)
Compatible Buffer
10X Buffer B
Sensitive to Heat Inactivation
Yes
Optimal Reaction Temperature
37°C
Type IIS RE
No

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