Thermo Scientific™

ScaI (10 U/µL)

Catalog number: ER0431
Thermo Scientific™

ScaI (10 U/µL)

Catalog number: ER0431
Catalog Number
ER0431
Unit Size
1,000 units
Price (USD)
73.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5,000 units
Price (USD)
303.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
ER04311,000 units
73.00
Your Price:
***
ER04325,000 units
303.00
Your Price:
***
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Thermo Scientific ScaI restriction enzyme recognizes AGT^ACT sites and cuts best at 37°C in its own unique buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. Isoschizomers: AssI, BmcAI, ZrmI.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features:

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note:
Conditions of low salt, high enzyme concentration, glycerol concentration > 5% or pH > 8.0 may result in star activity. Supercoiled plasmids may require up to 20-fold more ScaI for complete digestion than linear DNA.

For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity
1000 U
Methylation Sensitivity
Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive
Enzyme
Sca I
Compatible Buffer
Unique Buffer (10X Buffer ScaI)
Sensitive to Heat Inactivation
Yes
Optimal Reaction Temperature
37°C
Type IIS RE
No

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