Thermo Scientific™

KpnI, HC (50 U/µL)

Catalog number:  ER0523

Related applications:

Restriction Enzyme Cloning

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5'  G  G  T  A  C ↓C   3' 
3'  C ↑C  A  T  G  G   5' 

Thermo Scientific KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Isoschizomers: Asp718I.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.


• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities


• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)

Note: For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.


Quantity: HC; 20,000U
Methylation Sensitivity: Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive
Enzyme: Kpn I
Compatible Buffer: Unique Buffer (10x Buffer KpnI)
Sensitive to Heat Inactivation: Yes
Optimal Reaction Temperature: 37° C
Type IIS RE: No