Thermo Scientific™

BpiI (BbsI) (10 U/µL)

Catalog number: ER1011
Thermo Scientific™

BpiI (BbsI) (10 U/µL)

Catalog number: ER1011
Catalog Number
ER1011
Unit Size
200 units
Price (USD)
49.77
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1,000 units
Price (USD)
200.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
ER1011200 units
49.77
Your Price:
***
ER10121,000 units
200.00
Your Price:
***
Product Overview
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5'  G  A  A  G  A  C  N2 3'
3'  C  T  T  C  T  G  N6 5'

Thermo Scientific BpiI (BbsI) restriction enzyme recognizes GAAGAC(2/6)^ sites and cuts best at 37°C in G buffer (isoschizomers: BbsI, BpuAI, BstV2I). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: BpiI cleaves downstream of its recognition site and can generate any desired 4 base 5'-overhangs. This feature is useful for direct PCR product cloning. For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity
200 U
Methylation Sensitivity
Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive
Enzyme
BpiI (BbsI)
Compatible Buffer
10X Buffer G
Sensitive to Heat Inactivation
Yes
Optimal Reaction Temperature
37°C
Type IIS RE
No

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