Thermo Scientific™

ApaI (10 U/µL)

Catalog number: ER1411
Thermo Scientific™

ApaI (10 U/µL)

Catalog number: ER1411
Catalog Number
ER1411
Unit Size
3,000 units
Price (USD)
63.25
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5,000 units
Price (USD)
84.75
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
ER14113,000 units
63.25
Your Price:
***
ER14155,000 units
84.75
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
5'  G  G  G  C  C ↓C   3' 
3'  C ↑C  C  G  G  G   5' 

Thermo Scientific ApaI restriction enzyme recognizes GGGCC^C sites and cuts best at 37°C in B buffer (isoschizomers: PspOMI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Note: Incubation at 30°C results in a 2-fold increase in activity. ApaI is inhibited by salt concentrations above 50 mM. ApaI cleavage is impaired by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain, such as GM2163. For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Methylation Sensitivity
Not dam methylation-sensitive, dcm methylation-sensitive, CpG methylation-sensitive
Enzyme
Apa I
Compatible Buffer
10X Buffer B
Sensitive to Heat Inactivation
Yes
Optimal Reaction Temperature
37°C
Type IIS RE
No

Figures

Frequently asked questions (FAQs)

Citations & References