Thermo Scientific™

Nb.Mva1269I (10 U/µL)

Catalog number: ER2051
Thermo Scientific™

Nb.Mva1269I (10 U/µL)

Catalog number: ER2051
Catalog Number
ER2051
Unit Size
1,000 units
Price (USD)
78.75
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
ER20511,000 units
78.75
Your Price:
***
Product Overview
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5'  G  A  A  T  G  C   3' 
3'  C  T  T  A  C ↑G   5' 

Thermo Scientific Nb.Mva1269I nicking enzyme recognizes GAATGC sites and nicks best at 37°C in O buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications
Production of single-stranded circular DNA from supercoiled double-stranded plasmids in vitro with subsequent use in DNA sequencing, site-specific mutagenesis, etc Creation of nested deletions. Vector preparation for ligation independent cloning method Preparations of covalently closed, double-stranded linear DNA molecules.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Enzyme
Nb.Mva1269I
Compatible Buffer
10X Buffer O
Sensitive to Heat Inactivation
Yes
Optimal Reaction Temperature
37°C
Type IIS RE
No

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