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Thermo Scientific™

Lsp1109I (BbvI) (5 U/μL)

Catalog number: ER2071
Thermo Scientific™

Lsp1109I (BbvI) (5 U/μL)

Catalog number: ER2071
Catalog Number
ER2071
Unit Size
200 units
Price (USD)
Price: 63.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
ER2071200 units
Price: 63.00
Your price:
-
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5'  G  C  A  G  C  N8 3'
3'  C  G  T  C  G  N12 5'

Thermo Scientific Lsp1109I (BbvI) restriction enzyme recognizes GCAGC(8/12)^ sites and cuts best at 37°C in its own unique buffer (isoschizomers: BbvI, BstV1I). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: Assayed using pBR322 DNA (#SD0041). Lsp1109I may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis. Greater than 32-fold overdigestion with Lsp1109I may result in star activity. For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
5 U/μL
Methylation Sensitivity
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Enzyme
Lsp1109I (BbvI)
Compatible Buffer
Unique Buffer (10X Buffer Lsp1109I (BbvI))
Sensitive to Heat Inactivation
Yes
Optimal Reaction Temperature
37°C
Type IIS RE
Yes
Product Type
Restriction Enzyme
Research Category
Scarless Cloning

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