FilmTracer™ FM™ 1-43 Green Biofilm Cell Stain

Citas y referencias (4)

Invitrogen™

FilmTracer™ FM™ 1-43 Green Biofilm Cell Stain

Los colorantes FM son compuestos de estiril lipofílico utilizados en una amplia variedad de estudios que involucran membrana plasmática yMás información

Número de catálogo	Cantidad
F10317	1 unidad

Número de catálogo F10317

Precio (MXN)

Cantidad:

1 unidad

Los colorantes FM son compuestos de estiril lipofílico utilizados en una amplia variedad de estudios que involucran membrana plasmática y vesiculación. Los colorantes solubles en agua FM® son prácticamente no fluorescentes en medios acuosos y se cree que se insertan en la membrana de la superficie donde se vuelven intensamente fluorescentes. La tinción FM® 1-43 se ha utilizado con éxito para teñir los cuerpos celulares específicamente en una biopelícula compleja mileu, incluyendo Pseudomonas aeruginosa, Escherichia coli, Staphylococcus sp., Acidothiobacillus caldus y Vibrio cholerae.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorVerde

Método de detecciónFluorescente

Tipo de coloranteFM 1-43⁄lípido

EmisiónVisible

Intervalo de longitud de onda de excitación472⁄580

Para utilizar con (equipo)Microscopio confocal, microscopio de fluorescencia, citómetro de flujo, lector de microplacas, generador de imágenes de fluorescencia

FormularioSólido

Línea de productosFilmTracer, Molecular Probes

Cantidad1 unidad

Condiciones de envíoTemperatura ambiente

Tipo de etiquetaOtras etiquetas o colorantes

Tipo de productoTinte

SubCellular LocalizationMembranas celulares y lípidos, Lipids

Unit SizeEach

Contenido y almacenamiento

Almacenar a temperatura ambiente; desecar; proteger de la luz

Preguntas frecuentes

The product sheet for FM 1-43 Dye states that the maximum for excitation is 510 nm and emissions is 626 nm, but the spectrum on the product page shows a peak at 470 nm and 580 nm. Can you clarify what the excitation and emission maxima is for this dye?

FM lipophilic styryl dyes are known to shift their spectral properties upon binding to membranes. These dyes exhibit an appreciable solvent and environmental dependency in both free and membrane-bound forms. The emissions of the membrane-bound dye shifts to a shorter wavelength compared to the emission in the solvent.

In solvent, FM 1-43 Dye (Cat. Nos. T35356, T3163, F35355, F10317) absorbs in the range of fluorescein (~490 nm) and initially emits at around 626-636 nm. However, upon membrane integration, it exhibits emission in the 565-590 nm range. Because this dye exhibits a large Stokes shift, it does not perfectly fit into a green or orange fluorophore profile, but is rather a yellow-fluorescent dye, and may require a long-pass emission filter.

Note: FM 1-43 Dye is often used with typical fluorescein or GFP optical filter sets for biological imaging but is poorly excited through tetramethylrhodamine filters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (4)

Citations & References

Abstract

A novel role for enzyme I of the Vibrio cholerae phosphoenolpyruvate phosphotransferase system in regulation of growth in a biofilm.

Authors:Houot L, Watnick PI,

Journal:J Bacteriol

PubMed ID:17981973

Glucose is a universal energy source and a potent inducer of surface colonization for many microbial species. Highly efficient sugar assimilation pathways ensure successful competition for this preferred carbon source. One such pathway is the phosphoenolpyruvate phosphotransferase system (PTS), a multicomponent sugar transport system that phosphorylates the sugar as it ... More

Fluorescence staining of live cyanobacterial cells suggest non-stringent chromosome segregation and absence of a connection between cytoplasmic and thylakoid membranes.

Authors:Schneider D, Fuhrmann E, Scholz I, Hess WR, Graumann PL,

Journal:BMC Cell Biol

PubMed ID:17767716

BACKGROUND: In spite of their abundance and importance, little is known about cyanobacterial cell biology and their cell cycle. During each cell cycle, chromosomes must be separated into future daughter cells, i.e. into both cell halves, which in many bacteria is achieved by an active machinery that operates during DNA ... More

Genome Sequence and Composition of a Tolyporphin-Producing Cyanobacterium-Microbial Community.

Authors:

Journal:Appl Environ Microbiol

PubMed ID:28754701

Beta-Defensin 2 and 3 Promote Bacterial Clearance of Pseudomonas aeruginosa by Inhibiting Macrophage Autophagy through Downregulation of Early Growth Response Gene-1 and c-FOS.

Authors:

Journal:Front Immunol

PubMed ID:29487594