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Fluo-4 Direct™ Calcium Assay Kit
The Fluo-4 Direct™ Calcium Assay Kit was formulated to provide a homogeneous fluorescent calcium assay that: 1. Easily loads intoRead more
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| Catalog Number | Quantity |
|---|---|
| F10472 | 1 x 100 mL |
| F10471 | 10 x 10 mL |
| F10473 | 1 x 1 L |
Catalog number F10472
Price (USD)/ Each
623.65
Online Exclusive
666.00Save 42.35 (6%)
Quantity:
1 x 100 mL
Price (USD)/ Each
623.65
Online Exclusive
666.00Save 42.35 (6%)
The Fluo-4 Direct™ Calcium Assay Kit was formulated to provide a homogeneous fluorescent calcium assay that:
1. Easily loads into cells
2. Achieves a large assay window and
3. Suppresses background fluorescence generated from the calcium indicator in complete media with little-to-no impact on the specific cellular fluorescence
This advanced formulation allows the assay to be run in a simple “addition only” format in the presence of serum-containing media. Fluo-4 Direct™ is the third addition to the Fluo-4 family of calcium detection reagents. Fluo-4 AM and Fluo-4 NW both require media removal before assay detection, but Fluo-4 NW adds convenience by including the PowerLoad™ reagent for ease in cell loading. The Fluo-4 Direct™ Calcium Assay Kit is similar to Fluo-4 NW in that it is formulated with PowerLoad™ for ease in cell loading, but unique in that it can be used in the presence of complete culture media and will efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.
1. Easily loads into cells
2. Achieves a large assay window and
3. Suppresses background fluorescence generated from the calcium indicator in complete media with little-to-no impact on the specific cellular fluorescence
This advanced formulation allows the assay to be run in a simple “addition only” format in the presence of serum-containing media. Fluo-4 Direct™ is the third addition to the Fluo-4 family of calcium detection reagents. Fluo-4 AM and Fluo-4 NW both require media removal before assay detection, but Fluo-4 NW adds convenience by including the PowerLoad™ reagent for ease in cell loading. The Fluo-4 Direct™ Calcium Assay Kit is similar to Fluo-4 NW in that it is formulated with PowerLoad™ for ease in cell loading, but unique in that it can be used in the presence of complete culture media and will efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorGreen
Detection MethodFluorescence
For Use With (Application)Calcium Assay
For Use With (Equipment)Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
Product TypeCalcium Assay Kit
Dye TypeFluorescent Dye-Based
EmissionVisible
FormPowder
ModificationChemical
Product LineMolecular Probes™
Quantity1 x 100 mL
Shipping ConditionRoom Temperature
Sub Cellular LocalizationNucleus, Organelles, Cytoplasm
Unit SizeEach
Contents & Storage
- 100 ml Fluo-4 Direct™ Calcium Assay Reagent (Component A)
- 2 × 77 mg Probenecid (Component B)
- 200 ml Fluo-4 Direct™ Calcium Assay Buffer (Component C)
- Store at ≤-20°C. Dessicate and protect from light.