The Flow Cytometry Sub-micron Particle Size Reference Kit provides six sizes of green-fluorescent beads (ranging in diameter from 0.02 µm to 2.0 µm) packaged in individual vials. Each bead is uniform in size, and the green-fluorescent dyes are localized within the bead, which makes the beads brighter and more photostable than conventional surface-stained beads.
These size standards can help identify small bioparticles such as exosomes, bacteria, and viruses in experimental samples. This kit can also be used as a tool to verify instrument performance and establish parameters suitable for analyzing sub-micron particles, including: • Particle size resolution limit and dynamic range • Sensitivity of forward and side scatter photomultiplier tubes • Level of instrument baseline noise • Laser and optical alignment and stability • Stability of the fluidics system
The bead diameters are determined by transmission electron microscopy, and the beads are provided as 2 mL suspensions with a density of ~1× 106 beads/mL.