New ratiometric fluorescent calcium indicators with moderately attenuated binding affinities.
AuthorsGee KR, Archer EA, Lapham LA, Leonard ME, Zhou ZL, Bingham J, Diwu Z
JournalBioorg Med Chem Lett
PubMed ID10915039
Mono-halogenated derivatives of the calcium indicators fura-2 and indo-1 were synthesized and their spectroscopic properties evaluated. Halogenation ortho or para to the bridging oxygen in the BAPTA nucleus had a more pronounced weakening effect on binding affinity than in the meta position in the fura derivatives. Two new excitation ratioable ... More
Analysis of neurotransmitter release mechanisms by photolysis of caged Ca²? in an autaptic neuron culture system.
AuthorsBurgalossi A, Jung S, Man KN, Nair R, Jockusch WJ, Wojcik SM, Brose N, Rhee JS,
JournalNat Protoc
PubMed ID22722370
Neurotransmitter release is triggered by membrane depolarization, Ca(2+) influx and Ca(2+) sensing by the release machinery, causing synaptic vesicle (SV) fusion with the plasma membrane. Interlinked is a complex membrane cycle in which vesicles are tethered to the release site, primed, fused and recycled. As many of these processes are ... More
Calcium influx through receptor-operated channel induces mitochondria-triggered paraptotic cell death.
AuthorsJambrina E, Alonso R, Alcalde M, del Carmen Rodríguez M, Serrano A, Martínez-A C, García-Sancho J, Izquierdo M
JournalJ Biol Chem
PubMed ID12571238
We address the specific role of cytoplasmic Ca(2+) overload as a cell death trigger by expressing a receptor-operated specific Ca(2+) channel, vanilloid receptor subtype 1 (VR1), in Jurkat cells. Ca(2+) uptake through the VR1 channel, but not capacitative Ca(2+) influx stimulated by the muscarinic type 1 receptor, induced sustained intracellular ... More
The SNARE protein SNAP-25 is linked to fast calcium triggering of exocytosis.
AuthorsSørensen JB, Matti U, Wei SH, Nehring RB, Voets T, Ashery U, Binz T, Neher E, Rettig J
JournalProc Natl Acad Sci U S A
PubMed ID11830673
Synchronous neurotransmission depends on the tight coupling between Ca(2+) influx and fusion of neurotransmitter-filled vesicles with the plasma membrane. The vesicular soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein synaptobrevin 2 and the plasma membrane SNAREs syntaxin 1 and synaptosomal protein of 25 kDa (SNAP-25) are essential for calcium-triggered exocytosis. ... More
Characterization of a range of fura dyes with two-photon excitation.
AuthorsWokosin DL, Loughrey CM, Smith GL
JournalBiophys J
PubMed ID14990500
Two-photon excitation (TPE) spectra of Fura-2, -4F, -6F, -FF, and Furaptra were characterized using a tunable (750-850 nM) ultra-short pulse laser. Two-photon fluorescence of these dyes was studied in free solution and in the cytosol of isolated rabbit ventricular cardiomyocytes. The TPE spectra of the Ca(2+)-free and Ca(2+)-bound forms of ... More