A light-dependent increase in free Ca2+ concentration in the salamander rod outer segment.
AuthorsMatthews HR, Fain GL
JournalJ Physiol
PubMed ID11306652
'1. The Ca(2+) indicator dye fluo-5F was excited by an argon ion laser to measure changes in free Ca(2+) concentration ([Ca2+]i) in the outer segments of isolated salamander rods rapidly exposed to a 0 Ca(2+), 0 Na(+) solution designed to minimise surface membrane Ca(2+) fluxes. Over 30-60 s of laser ... More
Molecular and functional characterization of inositol trisphosphate receptors during early zebrafish development.
AuthorsAshworth R, Devogelaere B, Fabes J, Tunwell RE, Koh KR, De Smedt H, Patel S
JournalJ Biol Chem
PubMed ID17331947
'Fluctuations in cytosolic Ca(2+) are crucial for a variety of cellular processes including many aspects of development. Mobilization of intracellular Ca(2+) stores via the production of inositol trisphosphate (IP(3)) and the consequent activation of IP(3)-sensitive Ca(2+) channels is a ubiquitous means by which diverse stimuli mediate their cellular effects. Although ... More
Dopaminergic regulation of dendritic calcium: fast multisite calcium imaging.
AuthorsZhou WL, Oikonomou KD, Short SM, Antic SD,
JournalMethods Mol Biol
PubMed ID23296782
'Optimal dopamine tone is required for the normal cortical function; however it is still unclear how cortical-dopamine-release affects information processing in individual cortical neurons. Thousands of glutamatergic inputs impinge onto elaborate dendritic trees of neocortical pyramidal neurons. In the process of ensuing synaptic integration (information processing), a variety of calcium ... More
Nonlinear regulation of unitary synaptic signals by CaV(2.3) voltage-sensitive calcium channels located in dendritic spines.
AuthorsBloodgood BL, Sabatini BL
JournalNeuron
PubMed ID17224406
'The roles of voltage-sensitive sodium (Na) and calcium (Ca) channels located on dendrites and spines in regulating synaptic signals are largely unknown. Here we use 2-photon glutamate uncaging to stimulate individual spines while monitoring uncaging-evoked excitatory postsynaptic potentials (uEPSPs) and Ca transients. We find that, in CA1 pyramidal neurons in ... More
'Although action potentials are typically generated in the axon initial segment (AIS), the timing and pattern of action potentials are thought to depend on inward current originating in somatodendritic compartments. Using two-photon imaging, we show that T- and R-type voltage-gated Ca(2+) channels are colocalized with Na(+) channels in the AIS ... More
Genetic evidence for a protein-kinase-A-mediated presynaptic component in NMDA-receptor-dependent forms of long-term synaptic potentiation.
AuthorsHuang YY, Zakharenko SS, Schoch S, Kaeser PS, Janz R, Südhof TC, Siegelbaum SA, Kandel ER
JournalProc Natl Acad Sci U S A
PubMed ID15967982
'The synaptic vesicle protein Rab3A is a small GTP-binding protein that interacts with rabphilin and RIM1alpha, two presynaptic substrates of protein kinase A (PKA). Mice lacking RIM1alpha and Rab3A have a defect in PKA-dependent and NMDA receptor (NMDAR)-independent presynaptic long-term potentiation (LTP) at hippocampal mossy-fiber and cerebellar parallel-fiber synapses. In ... More
Synapse-specific plasticity and compartmentalized signaling in cerebellar stellate cells.
AuthorsSoler-Llavina GJ, Sabatini BL
JournalNat Neurosci
PubMed ID16680164
'Here we demonstrate that cerebellar stellate cells diffusionally isolate synaptically evoked signals in dendrites and are capable of input-specific synaptic plasticity. Sustained activity of parallel fibers induces a form of long-term depression that requires opening of calcium (Ca(2+))-permeable AMPA-type glutamate receptors (CP-AMPARs) and signaling through class 1 metabotropic glutamate receptors ... More
Optical induction of synaptic plasticity using a light-sensitive channel.
AuthorsZhang YP, Oertner TG
JournalNat Methods
PubMed ID17195846
We have combined millisecond activation of channelrhodopsin-2 (ChR2), a light-gated ion channel, with two-photon calcium imaging to investigate active synaptic contacts in rat hippocampal slice cultures. Calcium influx was larger during light-induced action potentials than during action potentials induced by somatic current injection, leading to highly reproducible synaptic transmission. Pairing ... More
The life cycle of Ca(2+) ions in dendritic spines.
AuthorsSabatini BL, Oertner TG, Svoboda K
JournalNeuron
PubMed ID11832230
Spine Ca(2+) is critical for the induction of synaptic plasticity, but the factors that control Ca(2+) handling in dendritic spines under physiological conditions are largely unknown. We studied [Ca(2+)] signaling in dendritic spines of CA1 pyramidal neurons and find that spines are specialized structures with low endogenous Ca(2+) buffer capacity ... More
Characterizing the conductance underlying depolarization-induced slow current in cerebellar Purkinje cells.
AuthorsKim YS, Kang E, Makino Y, Park S, Shin JH, Song H, Launay P, Linden DJ,
JournalJ Neurophysiol
PubMed ID23197456
Brief strong depolarization of cerebellar Purkinje cells produces a slow inward cation current [depolarization-induced slow current (DISC)]. Previous work has shown that DISC is triggered by voltage-sensitive Ca influx in the Purkinje cell and is attenuated by blockers of vesicular loading and fusion. Here, we have sought to characterize the ... More
Properties of ribbon and non-ribbon release from rod photoreceptors revealed by visualizing individual synaptic vesicles.
AuthorsChen M, Van Hook MJ, Zenisek D, Thoreson WB,
JournalJ Neurosci
PubMed ID23365244
Vesicle release from rod photoreceptors is regulated by Ca(2+) entry through L-type channels located near synaptic ribbons. We characterized sites and kinetics of vesicle release in salamander rods by using total internal reflection fluorescence microscopy to visualize fusion of individual synaptic vesicles. A small number of vesicles were loaded by ... More
Somatic spikes regulate dendritic signaling in small neurons in the absence of backpropagating action potentials.
AuthorsMyoga MH, Beierlein M, Regehr WG,
JournalJ Neurosci
PubMed ID19535592
Somatic spiking is known to regulate dendritic signaling and associative synaptic plasticity in many types of large neurons, but it is unclear whether somatic action potentials play similar roles in small neurons. Here we ask whether somatic action potentials can also influence dendritic signaling in an electrically compact neuron, the ... More
Dye loading with patch pipettes.
AuthorsEilers J, Konnerth A,
JournalCSH Protoc
PubMed ID20147143
This protocol describes the loading of individual cells with fluorescent probes via patch pipettes. The patch-clamp methodology has been successfully used for single-cell dye labeling in cultured neurons, brain slices, and in vivo preparations. A broad range of dyes can be used with this loading technique. Markers for morphological reconstruction ... More
The number of glutamate receptors opened by synaptic stimulation in single hippocampal spines.
AuthorsNimchinsky EA, Yasuda R, Oertner TG, Svoboda K
JournalJ Neurosci
PubMed ID14985448
The number of receptors opening after glutamate release is critical for understanding the sources of noise and the dynamic range of synaptic transmission. We imaged [Ca2+] transients mediated by synaptically activated NMDA receptors (NMDA-Rs) in individual spines in rat brain slices. We show that Ca2+ influx through single NMDA-Rs can ... More
SK channels and NMDA receptors form a Ca2+-mediated feedback loop in dendritic spines.
AuthorsNgo-Anh TJ, Bloodgood BL, Lin M, Sabatini BL, Maylie J, Adelman JP
JournalNat Neurosci
PubMed ID15852011
Small-conductance Ca(2+)-activated K(+) channels (SK channels) influence the induction of synaptic plasticity at hippocampal CA3-CA1 synapses. We find that in mice, SK channels are localized to dendritic spines, and their activity reduces the amplitude of evoked synaptic potentials in an NMDA receptor (NMDAR)-dependent manner. Using combined two-photon laser scanning microscopy ... More
Facilitation at single synapses probed with optical quantal analysis.
AuthorsOertner TG, Sabatini BL, Nimchinsky EA, Svoboda K
JournalNat Neurosci
PubMed ID12055631
Many synapses can change their strength rapidly in a use-dependent manner, but the mechanisms of such short-term plasticity remain unknown. To understand these mechanisms, measurements of neurotransmitter release at single synapses are required. We probed transmitter release by imaging transient increases in [Ca(2+)] mediated by synaptic N-methyl-D-aspartate receptors (NMDARs) in ... More
Dendritic spine heterogeneity determines afferent-specific Hebbian plasticity in the amygdala.
AuthorsHumeau Y, Herry C, Kemp N, Shaban H, Fourcaudot E, Bissière S, Lüthi A
JournalNeuron
PubMed ID15629707
Functional compartmentalization of dendrites is thought to underlie afferent-specific integration of neural activity in laminar brain structures. Here we show that in the lateral nucleus of the amygdala (LA), an area lacking apparent laminar organization, thalamic and cortical afferents converge on the same dendrites, contacting neighboring but morphologically and functionally ... More
The elementary unit of store-operated Ca2+ entry: local activation of CRAC channels by STIM1 at ER-plasma membrane junctions.
AuthorsLuik RM, Wu MM, Buchanan J, Lewis RS
JournalJ Cell Biol
PubMed ID16966423
The activation of store-operated Ca(2+) entry by Ca(2+) store depletion has long been hypothesized to occur via local interactions of the endoplasmic reticulum (ER) and plasma membrane, but the structure involved has never been identified. Store depletion causes the ER Ca(2+) sensor stromal interacting molecule 1 (STIM1) to form puncta ... More
Facilitation of L-type Ca2+ channels in dendritic spines by activation of beta2 adrenergic receptors.
AuthorsHoogland TM, Saggau P
JournalJ Neurosci
PubMed ID15456814
We studied the contribution of L-type Ca2+ channels to action potential-evoked Ca2+ influx in dendritic spines of CA1 pyramidal neurons and the modulation of these channels by the beta2 adrenergic receptor. Backpropagating action potentials (bAPs) (three at 50 Hz) were evoked by brief somatic current injections, and Ca2+ transients were ... More
Measurement of cytoplasmic calcium concentration in the rods of wild-type and transducin knock-out mice.
A 10 microm spot of argon laser light was focused onto the outer segments of intact mouse rods loaded with fluo-3, fluo-4 or fluo-5F, to estimate dark, resting free Ca(2+) concentration ([Ca(2+)](i)) and changes in [Ca(2+)](i) upon illumination. Dye concentration was adjusted to preserve the normal physiology of the rod, ... More
Regulation of neuronal excitation-transcription coupling by Kv2.1-induced clustering of somatic L-type Ca2+ channels at ER-PM junctions.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID34750263
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Authors
JournalCell Rep
PubMed ID32726631
Paradoxical hyperexcitability from NaV1.2 sodium channel loss in neocortical pyramidal cells.
Authors
JournalCell Rep
PubMed ID34348157
Mediodorsal and Ventromedial Thalamus Engage Distinct L1 Circuits in the Prefrontal Cortex.
Authors
JournalNeuron
PubMed ID33188733
Kv2.1 mediates spatial and functional coupling of L-type calcium channels and ryanodine receptors in mammalian neurons.
Authors
JournalElife
PubMed ID31663850
Direct translation of climbing fiber burst-mediated sensory coding into post-synaptic Purkinje cell dendritic calcium.
Authors
JournalElife
PubMed ID32985976
Double-Nanodomain Coupling of Calcium Channels, Ryanodine Receptors, and BK Channels Controls the Generation of Burst Firing.
Authors
JournalNeuron
PubMed ID29144974
Dendritic osmosensors modulate activity-induced calcium influx in oxytocinergic magnocellular neurons of the mouse PVN.
Authors
JournalElife
PubMed ID34250900
Convergent, functionally independent signaling by mu and delta opioid receptors in hippocampal parvalbumin interneurons.
Authors
JournalElife
PubMed ID34787079
Calcium dependence of neurotransmitter release at a high fidelity synapse.