Thermo Scientific™

Phire Plant Direct PCR Master Mix

Catalog number:  F160S

Related applications:

PCR

Back to top

Description

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material like complex polysaccharides, polyphenols and others.

Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species as well as fungi, bacteria and yeast. The kit includes a complete set of optimized reagents and detailed protocols making it an ideal choice for amplification of plant DNA.

Highlights

• Direct PCR—sample is added directly to PCR reaction, therefore there is no need for time-consuming and expensive DNA purification steps.
• Dilution & Storage protocol available—allows tens of PCR reactions from one tiny sample and allows re-testing
• Specially engineered Phire Hot Start II DNA polymerase—extremely short PCR protocol times
• Master Mix format with premixed gel loading dye—minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Hot Start: Built-In Hot Start
Includes Label or Dye: Yes
Quantity: 250 x 50μL rxns
For Use With (Application): Direct PCR
GC-Rich PCR Performance: Medium
No. of Reactions: 250 x 50 L Reactions
Overhang: Blunt
Polymerase: Phire Hot Start II DNA polymerase
Product Line: Phire
Product Type Specs: Direct PCR Master Mix
Reaction Format: SuperMix or Master Mix
Reaction Speed: Fast

Contents & storage

Contains:
• 2 x 1.25 mL Phire Plant Direct PCR Master Mix (2X)
• 5 mL Dilution Buffer
• 40 µL Control Primer Mix (25 µM each)
• 2 x 1.25 mL Water, nuclease- free
• 50 µg O'GeneRuler Express DNA Ladder (sufficient for 100 applications)

Store at -20°C.