Thermo Scientific™

Phusion Blood Direct PCR Master Mix

Catalog number:  F175S

Related applications:

PCR

Back to top

Description

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.

This accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Master Mix an ideal choice even for demanding applications, such as cloning. Phusion Blood Direct PCR Master Mix also allows analysis of blood samples with different preservatives (e.g., heparin, EDTA, citrate), storage conditions and from various species.

Highlights

• Direct PCR—sample is added directly to PCR reaction, therefore therese is no need for time-consuming and expensive DNA purification steps
• Dilution & Storage protocol available—allows tens of PCR reactions from one tiny sample and allows re-testing
• Specially engineered Phusion DNA polymerase—extremely short PCR protocol times
• Master Mix format with premixed gel loading dye—minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Hot Start: Built-In Hot Start
Includes Label or Dye: Yes
Quantity: 100 x 20μL rxns
GC-Rich PCR Performance: High
No. of Reactions: 100 x 20 L Reactions
Overhang: Blunt
Polymerase: Phusion Blood II DNA Polymerase
Product Line: Phusion
Product Type Specs: Direct PCR Master Mix
Reaction Format: SuperMix or Master Mix
Reaction Speed: Fast

Contents & storage

Contains:
• 1 mL Phusion Blood Direct PCR Master Mix (2X)
• 40 µL Universal Control Primer Mix
• 0.5 mL 50 mM EDTA
• 1.5 mL 50 mM MgCl2
• 0.5 mL DMSO
• 1.25 mL Water, nuclease-free
• 50 µg O'GeneRuler Express DNA Ladder (sufficient for 100 applications)

Store at -20°C.