Invitrogen™

FluoReporter™ lacZ Flow Cytometry Kit

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Description

The FluoReporter® lacZ Flow Cytometry Kit provides materials and protocols for quantitating β-galactosidase activity with fluorescein di-V-galactoside (FDG) in single cells using flow cytometry. This kit is accompanied by a license to practice patented techniques for loading FDG by hypotonic shock and improving retention of fluorescein in lacZ-positive cells.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay: β-Gal (lacZ) Assay
Detection Method: Fluorescent
Excitation/Emission: 491⁄514
Flow Cytometer Laser Lines: 488
Form: Solution
Format: Tube(s)
No. of Reactions: 50
Product Line: FluoReporter®
Shipping Condition: Room Temperature
Substrate: FDG
Substrate Properties: Chemical Substrate
Substrate Type: Beta-Gal Substrate
Target Enzyme: Beta-Galactosidase
For Use With (Equipment): Flow Cytometer

Contents & storage

Contains 1 vial FDG (0.5 mL, 20 mM in water/DMSO/ethanol, 8:1:1, v/v), 1 vial PETG (2.0 mL, 50 mM in water), chloroquine diphosphates (1.0 mL, 30 mM in water) and 1 vial of propidium iodide (1.0 mL, 150 µM in water).

Store in freezer (-5 to -30°C) and protect from light.