Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® luminescent FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are coupled with platinum or europium to produce a tracer with a long lifetime (platinum and europium microspheres exhibit decay times of >600 microseconds compared to <50 nanoseconds for conventional fluorophores). Platinum and europium luminescent FluoSpheres® beads are useful for detecting a low-level protein or DNA target in a tissue sample or on a membrane in time-gated fluorescence detection schemes.
In addition, the carboxylate surface modification is available to facilitate the coupling of various molecules and proteins to the surface of the bead.
Characteristics of the Carboxylate Coupling Surface Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.
Choices for FluoSpheres® Fluorescent Microspheres Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations: • Ten fluorescent colors • Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm • Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine • Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum
We’ll Make a Custom Microsphere Product for You We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.
For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Contents & storage
Store in refrigerator (2–8°C) and protect from light.