Invitrogen™

Fc OxyBURST™ Green Assay Reagent

Catalog number:  F2902

Related applications:

Cell Viability, Proliferation & Function

| Cellular Imaging

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Description

The Fc OxyBURST® Green assay reagent comprises bovine serum albumin (BSA) that has been covalently linked to dichlorodihydrofluorescein (H2DCF) and then complexed with a purified rabbit polyclonal anti-BSA antibody. When this immune complex binds to Fc receptors, the nonfluorescent H2DCF molecules are internalized within the phagovacuoloe and subsequently oxidized to green-fluorescent 2''7'-dichlorofluorescein.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Fluorescent
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Label or Dye: Fc OxyBURST™ assay green reagent
No. of Reactions: 25
Concentration: 3 mg⁄ml
Format: Tube(s), Slide(s)
Excitation/Emission: 488⁄523
Flow Cytometer Laser Lines: 488
Form: Solution
Indicator Properties: Fluorescent Dye-Based
Indicator: Free Radical Sensor
Shipping Condition: Wet Ice
Solubility: DMSO (Dimethylsulfoxide)
Product Line: OXYBURST™

Contents & storage

Contains 1 vial of Fc OxyBURST™ assay green reagent (suspension in PBS containing sodium azide).

Store in refrigerator (2–8°C) and protect from light.