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Zitierungen und Referenzen (14)
Invitrogen™

Fc OxyBURST™ Green Assay Reagent

Das Fc OxyBURST™ Green Assay-Reagenz besteht aus Rinderserumalbumin (BSA), das kovalent mit Dichlorodihydrofluorescein (H2DCF) verknüpft und anschließend mit einem gereinigtenWeitere Informationen
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KatalognummerMenge
F290225 Reaktionen
Katalognummer F2902
Preis (EUR)
481,65
Exklusiv online
642,00
Ersparnis 160,35 (25%)
Each
Menge:
25 Reaktionen
Preis (EUR)
481,65
Exklusiv online
642,00
Ersparnis 160,35 (25%)
Each
Das Fc OxyBURST™ Green Assay-Reagenz besteht aus Rinderserumalbumin (BSA), das kovalent mit Dichlorodihydrofluorescein (H2DCF) verknüpft und anschließend mit einem gereinigten polyklonalen Kaninchen-Anti-BSA-Antikörper komplexiert wurde. Wenn dieser Immunkomplex an Fc-Rezeptoren bindet, werden die nicht fluoreszierenden H2DCF-Moleküle innerhalb des Phagovacuoloe internalisiert und anschließend zu grün-fluoreszierendem 2''7'-Dichlorfluorescein oxidiert.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Konzentration3 mg⁄ml
NachweisverfahrenFluoreszent
FarbstofftypSensor für freie Radikale
FormLösung
FormatRöhrchen, Objektträger
Menge25 Reaktionen
VersandbedingungNasseis, Nasses Eis
LöslichkeitDMSO (Dimethylsulfoxid), DMSO (Dimethylsulfoxid)
Emission488⁄523
Zur Verwendung mit (Geräte)Fluoreszenzmikroskop, Durchflusszytometer
ProduktlinieOXYBURST
ProdukttypAssayreagenz
Unit SizeEach
Inhalt und Lagerung
Enthält 1 Fläschchen Fc OxyBURST™ Assay-Grünreagenz (Suspension in PBS mit Natriumazid). Im Kühlschrank (2–8 °C) aufbewahren und vor Licht schützen.

Zitierungen und Referenzen (14)

Zitierungen und Referenzen
Abstract
The phosphoinositide-binding protein p40phox activates the NADPH oxidase during FcgammaIIA receptor-induced phagocytosis.
Authors:Suh CI, Stull ND, Li XJ, Tian W, Price MO, Grinstein S, Yaffe MB, Atkinson S, Dinauer MC
Journal:J Exp Med
PubMed ID:16880255
'Superoxide produced by the phagocyte reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is essential for host defense. Enzyme activation requires translocation of p67(phox), p47(phox), and Rac-GTP to flavocytochrome b558 in phagocyte membranes. To examine the regulation of phagocytosis-induced superoxide production, flavocytochrome b558, p47(phox), p67(phox), and the FcgammaIIA receptor were expressed ... More
Severe impairment in early host defense against Listeria monocytogenes in mice deficient in acid sphingomyelinase.
Authors:Utermöhlen O, Karow U, Löhler J, Krönke M
Journal:J Immunol
PubMed ID:12594290
'The phagolysosomal compartment is crucial for the defense against infection with intracellular pathogens. Within this compartment, the TNF- and IFN-gamma-responsive acid sphingomyelinase (ASMase) generates the signaling molecule ceramide, resulting in the activation of proteases like cathepsin D. To investigate the possible role of ASMase as a mediator of the antibacterial ... More
Quantitative multiwell myeloid differentiation assay using dichlorodihydrofluorescein diacetate (H2DCF-DA) or dihydrorhodamine 123 (H2R123).
Authors:Trayner ID, Rayner AP, Freeman GE, Farzaneh F
Journal:J Immunol Methods
PubMed ID:7594627
'It is well established that the fluorescent probes dichlorodihydrofluorescein diacetate (H2DCF-DA) and dihydrorhodamine 123 (H2R123) can be used to detect the respiratory burst response of mature myeloid cells. We describe a simple, fast and quantitative assay for myeloid differentiation based on the oxidation of these probes, which can be performed ... More
A cytosolic calcium transient is not necessary for degranulation or oxidative burst in immune complex-stimulated neutrophils.
Authors:Seetoo KF, Schonhorn JE, Gewirtz AT, Zhou MJ, McMenamin ME, Delva L, Simons ER
Journal:J Leukoc Biol
PubMed ID:9307071
'Receptor-mediated activation of neutrophils (PMN) initiates possibly interdependent events, including a rapid transient increase in [Ca2+]i, implicated as a second messenger. To investigate whether this transient is required for eventual degranulation, PMN were incubated with an intracellular Ca2+ chelator (BAPTA), then exposed to chemotactic peptide [N-formyl-methionyl-leucyl-phenylalanine (fMLP)l with or without ... More
Fluorescent probes for living cells.
Authors:Johnson I
Journal:Histochem J
PubMed ID:10188922
'The functional characteristics of fluorescent probes used for imaging and measuring dynamic processes in living cells are reviewed. Initial consideration is given to general design requirements for delivery, targeting, detectability and fluorescence readout, and current technologies for attaining them. Discussion then proceeds to the more application-specific properties of intracellular ion ... More
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